Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse

Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse

Author Bortolini, Maria A. T. Autor UNIFESP Google Scholar
Shynlova, Oksana Google Scholar
Drutz, Harold P. Google Scholar
Castro, Rodrigo A. Autor UNIFESP Google Scholar
Girao, Manoel J. B. C. Autor UNIFESP Google Scholar
Lye, Stephen Google Scholar
Alarab, May Google Scholar
Institution Univ Toronto
Universidade Federal de São Paulo (UNIFESP)
Mt Sinai Hosp
Abstract Aims We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM-myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma-actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls. Methods: During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real-time RT-PCR. Results: MHY11 gene expression was down-regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). in the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). the gene expression of CALD1, ACTG2, and TPM1 was significantly down-regulated in vaginal tissue of healthy women after menopause (P < 0.05). Conclusion: Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109-114, 2012. (C) 2011 Wiley Periodicals, Inc.
Keywords actin
menopause
myosin
POP
smooth muscle
vagina
Language English
Sponsor Physician's Services Inc. Foundation (PSI), Toronto, Ontario, Canada
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-01-01
Published in Neurourology and Urodynamics. Malden: Wiley-Blackwell, v. 31, n. 1, p. 109-114, 2012.
ISSN 0733-2467 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 109-114
Origin http://dx.doi.org/10.1002/nau.21175
Access rights Closed access
Type Article
Web of Science ID WOS:000299372300020
URI http://repositorio.unifesp.br/handle/11600/34433

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