CYP2E1 RsaI and 96-bp insertion genetic polymorphisms associated with risk for colorectal cancer

CYP2E1 RsaI and 96-bp insertion genetic polymorphisms associated with risk for colorectal cancer

Author Silva, Tiago Donizetti da Autor UNIFESP Google Scholar
Felipe, Aledson Vitor Autor UNIFESP Google Scholar
Pimenta, Celia Aparecida Marques Autor UNIFESP Google Scholar
Barao, Katia Autor UNIFESP Google Scholar
Forones, Nora Manoukian Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We investigated a possible association between alcoholism, cigarette smoking, obesity and CYP2E1 RsaI and 96-bp insertion genetic polymorphisms with risk for colorectal cancer (CRC). Patients with CRC (70 women and 61 men) were matched for gender and age to 206 healthy controls. the mean age of the two groups was 62 years. Meat intake, cigarette smoking and alcohol drinking were assessed using a specific frequency questionnaire. the body mass index was also calculated. DNA was extracted from peripheral blood; RsaI polymorphism genotypes were evaluated by PCR-RFLP and 96-bp insertion genetic polymorphisms were evaluated by specific primers. the distributions of CYP2E1 RsaI c1/c1, c1/c2 and c2/c2 genotypes were 90.2, 9.2 and 0.6%, respectively, in controls and 83.9, 13.7 and 2.4% in CRC cases. Allele c2 was associated with increased risk for CRC [odds ratio (OR) = 1.88, 95% confidence interval (95%CI) = 1.02-3.45]. the CYP2E1 RsaI c2/c2 genotype was associated with an increased risk for rectal cancer (OR = 3.23, 95% CI = 1.26-9.03). the 96-bp insertion was slightly more frequent in the CRC group (9.3 vs 11.4%, P = 0.19), especially in females (6.4 vs 11.5%, P = 0.34). Smoking, alcohol drinking or high intake of red meat and CYP2E1 polymorphisms were not associated with increased risk for CRC. the 96-bp insertion was marginally more frequent (P = 0.07) in undernourished CRC subjects. We concluded that the risk for CRC is higher among individuals with allele c2. the CYP2E1 RsaI c2/c2 genotype was associated with an increased risk for rectal cancer.
Keywords CYP2E1
Polymorphism
Colorectal cancer
Language English
Date 2012-01-01
Published in Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3138-3145, 2012.
ISSN 1676-5680 (Sherpa/Romeo, impact factor)
Publisher Funpec-editora
Extent 3138-3145
Origin http://dx.doi.org/10.4238/2012.September.3.2
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000308817800135
URI http://repositorio.unifesp.br/handle/11600/34408

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account