Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

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dc.contributor.author Janz, Felipe de Lara
dc.contributor.author Debes, Adriana de Aguiar
dc.contributor.author Cavaglieri, Rita de Cassia
dc.contributor.author Duarte, Sergio Aloisio
dc.contributor.author Romao, Carolina Martinez
dc.contributor.author Moron, Antonio Fernandes [UNIFESP]
dc.contributor.author Zugaib, Marcelo
dc.contributor.author Bydlowski, Sergio Paulo
dc.date.accessioned 2016-01-24T14:17:37Z
dc.date.available 2016-01-24T14:17:37Z
dc.date.issued 2012-01-01
dc.identifier http://dx.doi.org/10.1155/2012/649353
dc.identifier.citation Journal of Biomedicine and Biotechnology. New York: Hindawi Publishing Corporation, 10 p., 2012.
dc.identifier.issn 1110-7243
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/34375
dc.description.abstract Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. in this concern, dimethyl sulfoxide (Me2SO) showed the best results. the freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Financiadora de Estudos e Projetos (FINEP)
dc.description.sponsorship Instituto Nacional de Ciencia e Tecnologia-Fluidos Complexos (INCT-FCx), Brazil
dc.format.extent 10
dc.language.iso eng
dc.publisher Hindawi Publishing Corporation
dc.relation.ispartof Journal of Biomedicine and Biotechnology
dc.rights Acesso aberto
dc.title Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Fundacao Prosangue Hemoctr São Paulo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Sch Med, Lab Genet & Mol Hematol LIM 31, BR-05403000 São Paulo, Brazil
dc.description.affiliation Fundacao Prosangue Hemoctr São Paulo, BR-05403000 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Med, Dept Gynecol & Obstet, BR-05403000 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Sch Med, Dept Obstet, BR-04021001 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Sch Med, Dept Obstet, BR-04021001 São Paulo, Brazil
dc.identifier.file WOS000304937000001.pdf
dc.identifier.doi 10.1155/2012/649353
dc.description.source Web of Science
dc.identifier.wos WOS:000304937000001



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