Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Author Janz, Felipe de Lara Google Scholar
Debes, Adriana de Aguiar Google Scholar
Cavaglieri, Rita de Cassia Google Scholar
Duarte, Sergio Aloisio Google Scholar
Romao, Carolina Martinez Google Scholar
Moron, Antonio Fernandes Autor UNIFESP Google Scholar
Zugaib, Marcelo Google Scholar
Bydlowski, Sergio Paulo Google Scholar
Institution Universidade de São Paulo (USP)
Fundacao Prosangue Hemoctr São Paulo
Universidade Federal de São Paulo (UNIFESP)
Abstract Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. in this concern, dimethyl sulfoxide (Me2SO) showed the best results. the freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios.
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Financiadora de Estudos e Projetos (FINEP)
Instituto Nacional de Ciencia e Tecnologia-Fluidos Complexos (INCT-FCx), Brazil
Date 2012-01-01
Published in Journal of Biomedicine and Biotechnology. New York: Hindawi Publishing Corporation, 10 p., 2012.
ISSN 1110-7243 (Sherpa/Romeo, impact factor)
Publisher Hindawi Publishing Corporation
Extent 10
Origin http://dx.doi.org/10.1155/2012/649353
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000304937000001
URI http://repositorio.unifesp.br/handle/11600/34375

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