Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins

Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins

Author Praxedes-Garcia, Priscila Autor UNIFESP Google Scholar
Cruz-Silva, Ilana Autor UNIFESP Google Scholar
Gozzo, Andrezza Justino Autor UNIFESP Google Scholar
Nunes, Viviane Abreu Google Scholar
Torquato, Ricardo Jose Autor UNIFESP Google Scholar
Tanaka, Aparecida Sadae Autor UNIFESP Google Scholar
Figueiredo-Ribeiro, Rita de Cassia Google Scholar
Gonzalez Gonzalez, Yamile Google Scholar
Araujo, Mariana da Silva Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Inst Bot São Paulo
Univ La Habana
Abstract Several proteins have been isolated from seeds of leguminous, but this is the first report that a protease was obtained from seeds of Caesalpinia echinata Lam., a tree belonging to the Fabaceae family. This enzyme was purified to homogeneity by hydrophobic interaction and anion exchange chromatographies and gel filtration. This 61-kDa serine protease (CeSP) hydrolyses H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide (K-m 55.7 mu M) in an optimum pH of 7.1, and this activity is effectively retained until 50 degrees C. CeSP remained stable in the presence of kosmotropic anions (PO43-, SO42-, and CH3COO-) or chaotropic cations (K+ and Na+). It is strongly inhibited by TLCK, a serine protease inhibitor, but not by E-64, EDTA or pepstatin A. the characteristics of the purified enzyme allowed us to classify it as a serine protease. the role of CeSP in the seeds cannot be assigned yet but is possible to infer that it is involved in the mobilization of seed storage proteins.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2012-01-01
Published in Scientific World Journal. New York: Hindawi Publishing Corporation, 8 p., 2012.
ISSN 1537-744X (Sherpa/Romeo, impact factor)
Publisher Hindawi Publishing Corporation
Extent 8
Origin http://dx.doi.org/10.1100/2012/562715
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000305735000001
URI http://repositorio.unifesp.br/handle/11600/34350

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