Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

Author Soares, Tatiane Sanches Autor UNIFESP Google Scholar
Watanabe, Renata Midori Okuda Autor UNIFESP Google Scholar
Lemos, Francisco Jose Alves Google Scholar
Tanaka, Aparecida Sadae Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Norte Fluminense
Abstract Trypsin-like enzymes play an important role in the Aedes aegypti digestive process. the trypsin-like enzymes present in adults were characterized previously, but little is known about trypsins in larvae. in the present work, we identified one of the trypsin enzymes from Ae. aegypti larval midgut using a library of trypsin gene fragments, which was the sequence known as AAEL005607 from the Ae. aegypti genome. Quantitative PCR analysis showed that AAEL005607 was transcribed in all larval instars, but it was not present in adult midgut. in order to confirm transcription data, the trypsin-like enzymes from 4th instar larvae of Ae. aegypti midgut were purified and sequenced. Purified trypsin showed identity with the amino-terminal sequence of AAEL005607, AAEL005609 and AAEL005614. These three trypsins have high amino acids identity, and could all be used as a template for the design of inhibitors. in conclusion, for the first time, digestive enzymes of 4th larval instar of Ae. aegypti were purified and characterized. the knowledge of digestive enzymes present in Ae. aegypti larvae may be helpful in the development of a larvicide. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Mosquito
Trypsin
BPTI
Larvae
Aedes
Serine protease inhibitor
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 05/03514-9
CNPq: 575829/2008-7
Date 2011-12-10
Published in Gene. Amsterdam: Elsevier B.V., v. 489, n. 2, p. 70-75, 2011.
ISSN 0378-1119 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 70-75
Origin http://dx.doi.org/10.1016/j.gene.2011.08.018
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000297569700002
URI http://repositorio.unifesp.br/handle/11600/34327

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