Concentration of Hyaluronic Acid in Human Vocal Folds in Young and Old Subjects

Concentration of Hyaluronic Acid in Human Vocal Folds in Young and Old Subjects

Author Korn, Gustavo Polacow Autor UNIFESP Google Scholar
Maciel Martins, Joao Roberto Autor UNIFESP Google Scholar
Park, Sung Woo Autor UNIFESP Google Scholar
Mendes, Aline Autor UNIFESP Google Scholar
Kobayashi, Elsa Yoko Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
De Biase, Noemi Grigoletto Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Objective. To compare and evaluate concentrations of hyaluronic acid (HA) in normal vocal folds of both young and elderly people of both sexes.Study Design. Experimental study.Setting. the research was carried out at the ENT, Head and Neck Surgery Department and the Pharmacology Institute of São Paulo Federal University.Subjects and Methods. Enzyme-linked immunosorbent assay plates coated with biotin-conjugated binding protein and streptavidin-europium conjugate were sequentially incubated with samples from the middle portion of the cover, vocal ligament, and vocal muscle of 19 young and 20 elderly subjects of both sexes. After the release of europium in an enhancement solution, final fluorescence was measured using a fluorometer.Results. Statistically significant comparisons: (1) all subject groups combined exhibited a higher HA concentration in the cover and ligament compared with the muscle samples; (2) women subjects had a significantly lower HA concentration in muscle compared with the ligament, and male subjects showed significantly lower HA in muscle compared with the cover; and (3) both genders of young subjects showed a higher HA concentration in ligament samples compared with the muscle.Conclusion. HA concentrations are higher in the cover and ligament compared with the muscle in both genders and age groups, and there is a higher HA concentration in ligament compared with the muscle of young subjects of both genders. HA levels in the cover samples of younger women showed great variability that may relate to ovarian hormone levels, reflecting different phases of the menstrual cycle.
Keywords vocal cords
hyaluronic acid
quantitative analysis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-12-01
Published in Otolaryngology-head and Neck Surgery. London: Sage Publications Ltd, v. 145, n. 6, p. 981-986, 2011.
ISSN 0194-5998 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Ltd
Extent 981-986
Access rights Closed access
Type Article
Web of Science ID WOS:000311799200019

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