Ultrastructure of Submandibular Salivary Glands of Mouse: TEM and HRSEM Observations

Ultrastructure of Submandibular Salivary Glands of Mouse: TEM and HRSEM Observations

Author Picoli, Lara Cristina Autor UNIFESP Google Scholar
Dias, Fernando Jose Google Scholar
Mardegan Issa, Joao Paulo Google Scholar
Ogawa, Koichi Google Scholar
Ciena, Adriano Google Scholar
Iyomasa, Mamie Mizusaki Google Scholar
Lopes, Ruberval Armando Google Scholar
Watanabe, Ii-Sei Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Fukuoka Univ
Abstract The fine structure of submandibular glands of mouse were analyzed using light microscopy (LM), high resolution scanning electron microscopy (HRSEM), and transmission electron microscopy (TEM) methods. for LM, the specimens were embedded in Spurr resin, stained by toluidin blue solutions. for TEM, the tissues of submandibular salivary glands were fixed with modified Karnovsky solution and postfixed with osmium tetroxide. for HRSEM, the tissues were fixed with 2% osmium tetroxide solution in 1/15M sodium phosphate buffer (pH 7.4). the samples were immersed successively in dymethylsulphoxide and freeze cracked. the maceration was made in diluted osmium tetroxide for 24-48 h. the samples were examined by high resolution scanning electron microscopy. the intracellular components of acinar and ductal cells revealed clearly the Golgi apparatus, rough endoplasmic reticulum, secretory granules, and mitochondria. the end bulbs of Golgi lamellae and flattened cisterns of rough endoplasmic reticulum showed the luminal surface. A few mitochondria were identified intermingling between the rough endoplasmic reticulum and the mitochondriales cristae in three-dimensional HRSEM images. Secretory granules were numerous and presented different sizes. Small granules of ribosomes were attached on cistern surface, measuring 20-25 nm in diameter. Numerous arranged microvilli were found on the luminal surface of secretory canaliculus. the contact surfaces of acinar cells revealed complicated interdigitations by cytoplasmic processes. the mitochondria of duct cells were disposed vertically and surrounded by basal infoldings of plasma membranes. Basement membrane showed a spongy-like structure having an irregular surface with various strands and meshes of fine collagen fibrils. Microsc. Res. Tech. 74: 1154-1160, 2011. (C) 2011 Wiley Periodicals, Inc.
Keywords submandibular salivary gland
Golgi apparatus
mitochondria
TEM
HRSEM
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-12-01
Published in Microscopy Research and Technique. Malden: Wiley-Blackwell, v. 74, n. 12, p. 1154-1160, 2011.
ISSN 1059-910X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1154-1160
Origin http://dx.doi.org/10.1002/jemt.21008
Access rights Closed access
Type Article
Web of Science ID WOS:000297859200013
URI http://repositorio.unifesp.br/handle/11600/34266

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