Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi

Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi

Author Lovato, Diogo Ventura Autor UNIFESP Google Scholar
Amino, Rogerio Autor UNIFESP Google Scholar
Gonzalez, Yamile Google Scholar
Miranda, Antonio Autor UNIFESP Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Tanaka, Aparecida Sadae Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ La Habana
Abstract Infestins are Kazal-type serine protease inhibitors described in the midgut of Triatoma infestans, Chagas disease vector. of all infestins, only infestin 1R (INF1R) does not control host blood coagulation, due to its inhibitory specificity for chymotrypsin-like proteases. We further investigated the effect of INF1R on cell infection by Trypanosoma cruzi. the importance of INF1R reactive site to inhibit T. cruzi cell invasion was confirmed using 1RSFTI, a synthetic cyclic peptide containing the inhibitor reactive site region hybridized to the Sunflower Trypsin Inhibitor-1 (SFTI-1). Our results suggest that INF1R efficiently inhibited parasite cell invasion. for the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion. (C) 2011 Elsevier Inc. All rights reserved.
Keywords Protease inhibitor
Subtilisin A
Triatoma infestans
Trypanosoma cruzi
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 05/03514-9
FAPESP: 02/12593-1
FAPESP: 09/50434-1
CNPq: 470070/2004-8
Date 2011-12-01
Published in Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 129, n. 4, p. 362-367, 2011.
ISSN 0014-4894 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 362-367
Origin http://dx.doi.org/10.1016/j.exppara.2011.09.002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000297777300006
URI http://repositorio.unifesp.br/handle/11600/34242

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