Characterization of the infective properties of a new genetic group of Trypanosoma cruzi associated with bats

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dc.contributor.author Maeda, Fernando Yukio [UNIFESP]
dc.contributor.author Alves, Renan Melatto [UNIFESP]
dc.contributor.author Cortez, Cristian [UNIFESP]
dc.contributor.author Lima, Fabio Mitsuo [UNIFESP]
dc.contributor.author Yoshida, Nobuko [UNIFESP]
dc.date.accessioned 2016-01-24T14:17:27Z
dc.date.available 2016-01-24T14:17:27Z
dc.date.issued 2011-12-01
dc.identifier http://dx.doi.org/10.1016/j.actatropica.2011.09.001
dc.identifier.citation Acta Tropica. Amsterdam: Elsevier B.V., v. 120, n. 3, p. 231-237, 2011.
dc.identifier.issn 0001-706X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/34238
dc.description.abstract A new genotype of Trypanosoma cruzi, associated with bats from anthropic areas, was recently described. Here we characterized a T. cruzi strain from this new genetic group, which could be a potential source of infection to humans. Metacyclic trypomastigotes (MT) of this strain, herein designated BAT, were compared to MT of well characterized CL and G strains, as regards the surface profile and infectivity toward human epithelial HeLa cells. BAT strain MT expressed gp82, the surface molecule recognized by monoclonal antibody 3F6 and known to promote CL strain invasion by inducing lysosomal exocytosis, as well as mucin-like molecules, but lacked gp90, which functions as a negative regulator of invasion in G strain. A set of experiments indicated that BAT strain internalization is gp82-mediated, and requires the activation of host cell phosphatidylinositol 3-kinase, protein kinase C and the mammalian target of rapamycin. MT of BAT strain were able to migrate through a gastric mucin layer, a property associated with p82 and relevant for oral infection. Gp82 was found to be a highly conserved molecule. Analysis of the BAT strain gp82 domain, containing the cell binding- and gastric mucin-binding sites, showed 91 and 93% sequence identity with G and CL strains, respectively. Hela cell invasion by BAT strain MT was inhibited by purified mucin-like molecules, which were shown to affect lysosome exocytosis required for MT internalization. Although MT of BAT strain infected host cells in vitro, they were less effective than G or CL strains in infecting mice either orally or intraperitoneally. (C) 2011 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 231-237
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Acta Tropica
dc.rights Acesso aberto
dc.subject Trypanosoma cruzi en
dc.subject New genotype en
dc.subject Metacyclic forms en
dc.subject Cell invasion en
dc.subject Bats en
dc.title Characterization of the infective properties of a new genetic group of Trypanosoma cruzi associated with bats en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2006/61450-0
dc.description.sponsorshipID CNPq: 301409/2007-2
dc.description.sponsorshipID CNPq: 470726/2007-5
dc.identifier.file WOS000297428400012.pdf
dc.identifier.doi 10.1016/j.actatropica.2011.09.001
dc.description.source Web of Science
dc.identifier.wos WOS:000297428400012



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