Positive association of the hepatic lipase gene polymorphism c.514C > T with estrogen replacement therapy response

Positive association of the hepatic lipase gene polymorphism c.514C > T with estrogen replacement therapy response

Author Pulchinelli, Alvaro Autor UNIFESP Google Scholar
Costa, Ana Maria Massad Autor UNIFESP Google Scholar
Carvalho, Cristina V. de Autor UNIFESP Google Scholar
Nogueira-de-Souza, Naiara Corrêa Autor UNIFESP Google Scholar
Haidar, Mauro Abi Autor UNIFESP Google Scholar
Andriolo, Adagmar Autor UNIFESP Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Hepatic lipase (HL), an enzyme present in the hepatic sinusoids, is responsible for the lipolysis of lipoproteins. Human HL contains four polymorphic sites: G-250A, T-710C, A-763G, and C-514T single-nucleotide polymorphism (SNPs). the last polymorphism is the focus of the current study. the genotypes associated with the C-514T polymorphism are CC (normal homozygous - W), CT (heterozygous - H), and TT (minor-allele homozygous - M). HL activity is significantly impaired in individuals of the TT and CT genotypes. A total of 58 post-menopausal women were studied. the subjects were hysterectomized women receiving hormone replacement therapy consisting of 0.625 mg of conjugated equine estrogen once a day. the inclusion criteria were menopause of up to three years and normal blood tests, radiographs, cervical-vaginal cytology, and densitometry. DNA was extracted from the buccal and blood cells of all 58 patients using a commercially available kit (GFX (R) - Amersham-Pharmacia, USA).Results: Statistically significant reductions in triglycerides (t = 2.16; n = 58; p = 0.03) but not in total cholesterol (t = 0.14; n = 58; p = 0.89) were found after treatment. This group of good responders were carriers of the T allele; the CT and TT genotypes were present significantly more frequently than in the group of non-responders (p = 0.02 or p = 0.07, respectively). However, no significant difference in HDL-C (t = 0.94; n = 58; p = 0.35) or LDL-C (t = -0.83; n = 58; p = 0.41) was found in these patients.Conclusions: the variation in lipid profile associated with the C-514T polymorphism is significant, and the T allele is associated with the best response to ERT.
Keywords Hepatic lipase gene
Menopause
Hormone replacement therapy
Lipid metabolism
SNP
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-11-02
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 10, 9 p., 2011.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 9
Origin http://dx.doi.org/10.1186/1476-511X-10-197
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000297404300001
URI http://repositorio.unifesp.br/handle/11600/34221

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