Effect of dexamethasone on human osteoblasts in culture: involvement of beta 1 integrin and integrin-linked kinase

Effect of dexamethasone on human osteoblasts in culture: involvement of beta 1 integrin and integrin-linked kinase

Author Naves, Marcelo A. Autor UNIFESP Google Scholar
Pereira, Rosa M. R. Google Scholar
Comodo, Andreia N. Autor UNIFESP Google Scholar
Alvarenga, Erika L. F. C. de Autor UNIFESP Google Scholar
Caparbo, Valeria F. Google Scholar
Teixeira, Vicente P. C. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Adhesive interactions play a critical role in cell biology, influencing vital processes from proliferation to cell death. Integrins regulate cell-ECM (extracellular matrix) adhesion and must associate with phosphorylating proteins such as ILK (integrin-linked kinase). Dysregulation of ILK expression is associated with anchorage-independent growth, cell survival and inhibition of apoptosis. Glucocorticoids influence differentiation and adhesion of osteoblasts and can affect bone protein synthesis. the objective of this study was to analyse the effect of DEX (dexamethasone) on the biology of osteoblasts, together with its influence on the expression of ILK and beta 1 integrin. for this, primary cultures of human osteoblasts were exposed to DEX at 10(-9) M (physiological dose) and 10(-6) M (pharmacological dose) for 24 and 48 h. Cell viability, apoptosis and cell adhesion were analysed, as well as protein expression of beta 1 integrin and ILK. It was observed that cell viability and adhesion were reduced in the cultures evaluated. in comparison with the control cultures, there was slightly less apoptosis in the cultures exposed to the physiological dose and considerably more apoptosis in those exposed to the pharmacological dose. in all treated cultures, protein expression of ILK was slightly higher than in the control cultures, whereas that of beta 1 integrin was significantly lower. Both proteins under study were co-localized at the cell periphery in all cultures. Our results suggest that DEX causes osteoblast anoikis, probably due to decreased beta 1 integrin expression, which might have had a direct influence upon ILK, reducing its activation and preventing it from playing its characteristic antiapoptotic role.
Keywords adhesion ability
glucocorticoid
integrin-linked kinase (ILK)
osteoblast
primary cell culture
beta 1 integrin
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 07/54403-8
Date 2011-11-01
Published in Cell Biology International. Hoboken: Wiley-Blackwell, v. 35, n. 11, p. 1147-1151, 2011.
ISSN 1065-6995 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1147-1151
Origin http://dx.doi.org/10.1042/CBI20100731
Access rights Closed access
Type Article
Web of Science ID WOS:000297086100010
URI http://repositorio.unifesp.br/handle/11600/34206

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