Effects of adrenal hormones on the expression of adiponectin and adiponectin receptors in adipose tissue, muscle and liver

Effects of adrenal hormones on the expression of adiponectin and adiponectin receptors in adipose tissue, muscle and liver

Author Oliveira, Cristiane de Autor UNIFESP Google Scholar
Iwanaga-Carvalho, Carla Google Scholar
Mota, Joao F. Autor UNIFESP Google Scholar
Oyama, Lila M. Autor UNIFESP Google Scholar
Ribeiro, Eliane B. Autor UNIFESP Google Scholar
Oiler do Nascimento, Claudia M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Ctr Univ Cidade Rio de Janeiro
Abstract Background: Adiponectin, an insulin-sensitive hormone that is primarily synthesized in adipose tissue, exerts its effects by binding to two receptors, adipoR1 and adipoR2. Little is known regarding the effects of glucocorticoids on the expression of adiponectin receptors.Methods: Male Wistar rats were bilaterally adrenalectomized and treated with dexamethasone (0.2 mg/100 g) twice daily for 3 days. To analyze the potential effects of glucocorticoids, rats received two daily injections of the glucocorticoid receptor antagonist (RU-486, 5.0 mg) over the course of 3 days. Additionally, 3T3-L1 adipocytes and C2C12 myotubes were treated with dexamethasone, adrenaline or RU-486. the gene expression of adiponectin, adipoR1 and adipoR2 was determined by real-time PCR, and protein secretion was examined by Western blotting using lysates from retroperitoneal, epididymal and subcutaneous adipose tissue depots, liver and muscle.Results: in rats, excess glucocorticoids increased the levels of insulin in serum and decreased serum adiponectin concentrations, whereas adrenalectomy decreased the mRNA expression of adiponectin (3-fold) and adipoR2 (7-fold) in epididymal adipose tissue and increased adipoR2 gene expression in muscle (3-fold) compared to control group sham-operated. Dexamethasone treatment did not reverse the effects of adrenalectomy, and glucocorticoid receptor blockade did not reproduce the effects of adrenalectomy. in 3T3-L1 adipocytes, dexamethasone and adrenaline both increased adipoR2 mRNA levels, but RU-486 reduced adipoR2 gene expression in vitro.Conclusion: Dexamethasone treatment induces a state of insulin resistance but does not affect adiponectin receptor expression in adipose tissue. However, the effects of catecholamines on insulin resistance may be due to their effects on adipoR2. (C) 2011 Elsevier Inc. All rights reserved.
Keywords Adrenalectomy
Glucocorticoids
Catecholamines
Adiponectin receptors
Insulin resistance
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-11-01
Published in Steroids. New York: Elsevier B.V., v. 76, n. 12, p. 1260-1267, 2011.
ISSN 0039-128X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1260-1267
Origin http://dx.doi.org/10.1016/j.steroids.2011.06.004
Access rights Closed access
Type Article
Web of Science ID WOS:000295745500006
URI http://repositorio.unifesp.br/handle/11600/34202

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