Oxidative stress protection of Trypanosomes requires selenophosphate synthase

Oxidative stress protection of Trypanosomes requires selenophosphate synthase

Author Costa, Fernanda Cristina Google Scholar
Oliva, Maria Angela V. Google Scholar
Jesus, Teresa Cristina Leandro de Autor UNIFESP Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Thiemann, Otávio Henrique Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Selenoproteins are characterized by the incorporation of at least one amino acid selenocysteine (Sec-U) encoded by in-frame UGA stop codons. These proteins, as well as the components of the Sec synthesis pathway, are present in members of the bacteria, archaea and eukaryote domains. Although not a ubiquitous pathway in all organisms, it was also identified in several protozoa, including the Kinetoplastida. Genetic evidence has indicated that the pathway is non-essential to the survival of Trypanosoma growing in non-stressed conditions. By analyzing the effects of RNA interference of the Trypanosoma brucei selenophosphate synthetase SPS2, we found a requirement under sub-optimal growth conditions. the present work shows that SPS2 is involved in oxidative stress protection of the parasite and its absence severely hampers the parasite survival in the presence of an oxidizing environment that results in an apoptotic-like phenotype and cell death. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Trypanosoma
Selenocysteine
Selenophosphate synthetase
RNA interference
Oxidative stress
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 99/02874-9
FAPESP: 98/14138-2
FAPESP: 07/54621-5
CNPq: 480648/2007-7
CNPq: 472946/2007-2
Date 2011-11-01
Published in Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 180, n. 1, p. 47-50, 2011.
ISSN 0166-6851 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 47-50
Origin http://dx.doi.org/10.1016/j.molbiopara.2011.04.007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000295545000008
URI http://repositorio.unifesp.br/handle/11600/34160

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