Etanercept attenuates TNBS-induced experimental colitis: role of TNF-alpha expression

Etanercept attenuates TNBS-induced experimental colitis: role of TNF-alpha expression

Author Ribeiro Paiotti, Ana Paula Autor UNIFESP Google Scholar
Miszputen, Sender Jankiel Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Artigiani Neto, Ricardo Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Franco, Marcello Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Crohn's disease (CD) is associated with gut barrier dysfunction. Tumour necrosis factor-alpha (TNF-alpha) plays an important role into the pathogenesis of several inflammatory diseases because its expression is increased in inflamed mucosa of CD patients. Anti-TNF therapy improves significantly mucosal inflammation. Thus, this study aimed to evaluate the effect of Etanercept (ETC), a tumour necrosis factor alpha (TNF-alpha) antagonist on the 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis. A total of 18 Wistar rats were randomized into four groups, as follows: (1) Sham: sham induced-colitis; (2) TNBS: non-treated induced-colitis; (3) ETC control; (4) ETC-treated induced-colitis. Rats from group 4 presented significant improvement either of macroscopic or of histopathological damage in the distal colon. the gene expression of TNF-alpha mRNA, decreased significantly in this group compared to the TNBS non-treated group. the treatment with etanercept attenuated the colonic damages and reduced the inflammation caused by TNBS. Taken together, our results suggest that ETC attenuates intestinal colitis induced by TNBS in Wistar rats by TNF-alpha downregulation.
Keywords Inflammatory bowel disease
TNBS-colitis
TNF-alpha
Etanercept
RT-PCR
Language English
Date 2011-10-01
Published in Journal of Molecular Histology. Dordrecht: Springer, v. 42, n. 5, p. 443-450, 2011.
ISSN 1567-2379 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 443-450
Origin http://dx.doi.org/10.1007/s10735-011-9349-z
Access rights Closed access
Type Article
Web of Science ID WOS:000294824500008
URI http://repositorio.unifesp.br/handle/11600/34095

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