Hysteretic Behavior of Proprotein Convertase 1/3 (PC1/3)

Hysteretic Behavior of Proprotein Convertase 1/3 (PC1/3)

Author Icimoto, Marcelo Y. Autor UNIFESP Google Scholar
Barros, Nilana M. Google Scholar
Ferreira, Juliana C. Autor UNIFESP Google Scholar
Marcondes, Marcelo F. Autor UNIFESP Google Scholar
Andrade, Douglas Autor UNIFESP Google Scholar
Machado, Mauricio F. Autor UNIFESP Google Scholar
Juliano, Maria A. Autor UNIFESP Google Scholar
Judice, Wagner A. Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Mogi das Cruzes
Abstract The proprotein convertases (PCs) are calcium-dependent proteases responsible for processing precursor proteins into their active forms in eukariotes. the PC1/3 is a pivotal enzyme of this family that participates in the proteolytic maturation of prohormones and neuropeptides inside the regulated secretory pathway. in this paper we demonstrate that mouse proprotein convertase 1/3 (mPC1/3) has a lag phase of activation by substrates that can be interpreted as a hysteretic behavior of the enzyme for their hydrolysis. This is an unprecedented observation in peptidases, but is frequent in regulatory enzymes with physiological relevance. the lag phase of mPC1/3 is dependent on substrate, calcium concentration and pH. This hysteretic behavior may have implications in the physiological processes in which PC1/3 participates and could be considered an additional control step in the peptide hormone maturation processes as for instance in the transformation of proinsulin to insulin.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-09-15
Published in Plos One. San Francisco: Public Library Science, v. 6, n. 9, 7 p., 2011.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 7
Origin http://dx.doi.org/10.1371/journal.pone.0024545
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000295041700041
URI http://repositorio.unifesp.br/handle/11600/34049

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