Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli

Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli

Autor Menezes, Caroline A Google Scholar
Imamura, Sergio Y Google Scholar
Trabulsi, Luiz Rachid Google Scholar
Fernandes-Filho, Antônio Google Scholar
Martinez, Marina B Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Girão, Dennys M Google Scholar
Piazza, Roxane Mf Google Scholar
Instituição Instituto Butantan Laboratório de Bacteriologia
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro Instituto Prof. Paulo de Góes Departamento de Microbiologia Médica
Resumo Strains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterotoxin genes, and subsequent serotyping, but most clinical and public health laboratories do not have the capacity or resources to perform these tests. In this study, polyclonal rabbit and monoclonal mouse IgG2b antibodies against ETEC heat-labile toxin-I (LT) were characterized and the potential applicability of a capture assay was analyzed. IgG-enriched fractions from rabbit polyclonal and the IgG2b monoclonal antibodies recognized LT in a conformational shape and they were excellent tools for detection of LT-producing strains. These findings indicate that the capture immunoassay could be used as a diagnostic assay of ETEC LT-producing strains in routine diagnosis and in epidemiological studies of diarrhea in developing countries as enzyme linked immunosorbent assay techniques remain as effective and economical choice for the detection of specific pathogen antigens in cultures.
Palavra-chave detection
antibodies
heat-labile toxin
Escherichia coli
Idioma Inglês
Data de publicação 2006-12-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 101, n. 8, p. 875-880, 2006.
ISSN 0074-0276 (Sherpa/Romeo, fator de impacto)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 875-880
Fonte http://dx.doi.org/10.1590/S0074-02762006000800009
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000243649600009
SciELO S0074-02762006000800009 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/3402

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