Modeling epileptogenesis and temporal lobe epilepsy in a non-human primate

Modeling epileptogenesis and temporal lobe epilepsy in a non-human primate

Author Perez-Mendes, P. Autor UNIFESP Google Scholar
Blanco, M. M. Autor UNIFESP Google Scholar
Calcagnotto, M. E. Autor UNIFESP Google Scholar
Cinini, S. M. Autor UNIFESP Google Scholar
Bachiega, J. Autor UNIFESP Google Scholar
Papoti, D. Autor UNIFESP Google Scholar
Covolan, Luciene Autor UNIFESP Google Scholar
Tannus, A. Autor UNIFESP Google Scholar
Mello, L. E. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Here we describe a new non-human primate model of temporal lobe epilepsy (TLE) to better investigate the cause/effect relationships of human TLE. Status epilepticus (SE) was induced in adult marmosets by pilocarpine injection (250 mg/kg; i.p.). the animals were divided in 2 groups: acute (8 h post-SE) and chronic (3 and 5 months post-SE). To manage the severity of SE, animals received diazepam 5 min after the SE onset (acute group: 2.5 or 1.25 mg/kg; i.p.; chronic group/; 1.25 mg/kg; i.p). All animals were monitored by video and electrocorticography to assess SE and subsequent spontaneous recurrent seizures (SRS). To evaluate brain injury produced by SE or SRS we used argyrophil III, Nissl and neo-Timm staining techniques. Magnetic resonance image was also performed in the chronic group. We observed that pilocarpine was able to induce SE followed by SRS after a variable period of time. Prolonged SE episodes were associated with brain damage, mostly confined to the hippocampus and limbic structures. Similar to human TLE, anatomical disruption of dentate gyrus was observed after SRS. Our data suggest that pilocarpine marmoset model of epilepsy has great resemblance to human TLE, and could provide new tools to further evaluate the subtle changes associated with human epilepsy. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Pilocarpine
Temporal lobe epilepsy
Status epilepticus
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-09-01
Published in Epilepsy Research. Amsterdam: Elsevier B.V., v. 96, n. 1-2, p. 45-57, 2011.
ISSN 0920-1211 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 45-57
Access rights Closed access
Type Article
Web of Science ID WOS:000295706700006

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