Infection of retinal epithelial cells with L. amazonensis impacts in extracellular matrix proteins

Infection of retinal epithelial cells with L. amazonensis impacts in extracellular matrix proteins

Autor Calabrese, Katia da Silva Google Scholar
Silva, Leandro de Souza Google Scholar
Pereira Carvalho, Luiz Otavio Google Scholar
Hardoim, Daiana de Jesus Google Scholar
Silva-Almeida, Mariana da Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Freitas de Souza, Celeste da Silva Google Scholar
Instituição Fiocruz MS
Universidade Federal de São Paulo (UNIFESP)
Resumo One of the manifestations of leishmaniases is eye injuries which main characteristics are the injury of the anterior chamber of the eye and the resistance to specific treatments. the retinal pigment epithelial (RPE) cells participate in pathogen-induced intraocular inflammatory processes. We investigated Leishmania amazonensis-RPE cells relationship and its impact in laminin and fibronectin production. Using RPE cell (ARPE-19), we demonstrated that L. amazonensis adhere to these cells in the first hour of infection, whereas parasite internalization was only observed after 6 h. Seventy-two hours after infection, vacuoles with parasites debris were observed intracellularly, and no parasite were observed intra- or extracellularly at the 96 h, suggesting that Leishmania can infect ARPE-19 cells although this cells are able to clear the infection. Fibronectin and laminin were associated with L. amazonensis-ARPE-19 interaction. Confocal analysis showed no substantial alterations in fibronectin presence in ARPE-19-infected or ARPE-19-noninfected cells, whereas laminin levels increased three times 10 h after L. amazonensis infection. After this time, laminin levels decreased in infected cells. These results suggest that L. amazonensis-ARPE-19 infection induces increased production of laminin in the beginning of infection which may facilitate parasite-host cell interactions.
Idioma Inglês
Financiador Instituto Oswaldo Cruz
Data 2011-09-01
Publicado em Parasitology Research. New York: Springer, v. 109, n. 3, p. 727-736, 2011.
ISSN 0932-0113 (Sherpa/Romeo, fator de impacto)
Editor Springer
Extensão 727-736
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000294225700024

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