Endurance exercise training ameliorates insulin resistance and reticulum stress in adipose and hepatic tissue in obese rats

Endurance exercise training ameliorates insulin resistance and reticulum stress in adipose and hepatic tissue in obese rats

Author Luz, Gabrielle da Google Scholar
Frederico, Marisa J. S. Google Scholar
Silva, Sabrina da Google Scholar
Vitto, Marcelo F. Google Scholar
Cesconetto, Patricia A. Google Scholar
Pinho, Ricardo A. de Google Scholar
Pauli, Jose R. Autor UNIFESP Google Scholar
Silva, Adelino S. R. Google Scholar
Cintra, Dennys E. Google Scholar
Ropelle, Eduardo R. Google Scholar
De Souza, Claudio T. Google Scholar
Institution Univ Extremo Catarinense
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
Abstract Obesity-induced endoplasmatic reticulum (ER) stress has been demonstrated to underlie the induction of obesity-induced JNK and NF-kappa B activation inflammatory responses, and generation of peripheral insulin resistance. On the other hand, exercise has been used as a crucial tool in obese and diabetic patients, and may reduce inflammatory pathway stimulation. However, the ability of exercise training to reverse endoplasmatic reticulum stress in adipose and hepatic tissue in obesity has not been investigated in the literature. Here, we demonstrate that exercise training ameliorates ER stress and insulin resistance in DIO-induced rats. Rats were fed with standard rodent chow (3,948 kcal kg(-1)) or high-fat diet (5,358 kcal kg(-1)) for 2 months. After that rats were submitted to swimming training (1 h per day, 5 days for week with 5% overload of the body weight for 8 weeks). Samples from epididymal fat and liver were obtained and western blot analysis was performed. Our results showed that swimming protocol reduces pro-inflammatory molecules (JNK, I kappa B and NF-kappa B) in adipose and hepatic tissues. in addition, exercise leads to reduction in ER stress, by reducing PERK and eIF2 alpha phosphorylation in these tissues. in parallel, an increase in insulin pathway signaling was observed, as confirmed by increases in IR, IRSs and Akt phosphorylation following exercise training in DIO rats. Thus, results suggest that exercise can reduce ER stress, improving insulin resistance in adipose and hepatic tissue.
Keywords ER stress
Insulin resistance
Exercise training
Inflammatory pathway
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-09-01
Published in European Journal of Applied Physiology. New York: Springer, v. 111, n. 9, p. 2015-2023, 2011.
ISSN 1439-6319 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 2015-2023
Origin http://dx.doi.org/10.1007/s00421-010-1802-2
Access rights Closed access
Type Article
Web of Science ID WOS:000294963000007
URI http://repositorio.unifesp.br/handle/11600/34001

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