Role of vascular Kinin B-1 and B-2 receptors in endothelial nitric oxide metabolism

Role of vascular Kinin B-1 and B-2 receptors in endothelial nitric oxide metabolism

Author Loiola, Rodrigo Azevedo Autor UNIFESP Google Scholar
Reis, Felipe Castellani Gomes dos Autor UNIFESP Google Scholar
Mári-Kawamoto, Elisa Autor UNIFESP Google Scholar
Scavone, Cristoforo Autor UNIFESP Google Scholar
Abdalla, Dulcineia Saes Parra Autor UNIFESP Google Scholar
Fernandes, Liliam Autor UNIFESP Google Scholar
Pesquero, João Bosco Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Kinin B-1 and B-2 receptors play an essential role in inflammatory process and cardiovascular homeostasis. the present study investigated the vascular reactivity and nitric oxide (NO) generation in the isolated mesenteric arteriolar bed from B-1 (B-1(-/-)) and B-2 receptor (B-2(-/-)) knockout mice. Endothelial-dependent relaxation was significantly decreased in arterioles from both B-1(-/-) and B-2(-/-) in comparison to wild type (WT) mice, with no differences for endothelial-independent relaxating or vasoconstrictor agents. Plasmatic and vascular NO production were markedly reduced in both B-1(-/-) and B-2(-/-). in contrast, in the presence of L-arginine, Ca2+ and co-factors for the enzyme, NO synthase activity was higher in homogenates of mesenteric vessels of B-1(-/-) and B-2(-/-). the present study demonstrated that targeted deletion of B-1 or B-2 receptor gene in mice induces important alterations in the vascular reactivity of resistance vessels and NO metabolism. the severe impairment in the endothelial-mediated vasodilation accompanied by decreased NO bioavailability, despite the augmented NOS activity, strongly indicates an exacerbation of NO inactivation in B-1(-/-) and B-2(-/-) vessels. the present data provide valuable information in order to clarify the relevance of kinin receptors in regulating vascular physiology and may point to new approaches regarding its correlation with endothelial dysfunction, oxidative stress and NO availability. (C) 2011 Elsevier Inc. All rights reserved.
Keywords B-1 receptor
B-2 receptor
Knockout
Bradykinin
Vascular reactivity
Nitric oxide
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2007/59039-2
FAPESP: 2008/06676-8
Date 2011-08-01
Published in Peptides. New York: Elsevier B.V., v. 32, n. 8, p. 1700-1705, 2011.
ISSN 0196-9781 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1700-1705
Origin http://dx.doi.org/10.1016/j.peptides.2011.06.010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000294572800018
URI http://repositorio.unifesp.br/handle/11600/33897

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