Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development

Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development

Author Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Duailibi Neto, Eduardo Felippe Google Scholar
Negreiros, Renata Matalon Google Scholar
Jorge, Waldyr Antonio Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Vacanti, Joseph Phillip Google Scholar
Yelick, Pamela Crotty Google Scholar
Institution Tufts Univ
Universidade Federal de São Paulo (UNIFESP)
Natl Inst Sci & Technol
Universidade de São Paulo (USP)
Massachusetts Gen Hosp
Dept Surg
Harvard Univ
Abstract Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). the greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.
Keywords Tooth development
Tooth tissue engineering
Human dental stem cells
Human tooth development
Tooth rapid prototyping
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 07-58856-7
FAPESP: 07-51227-4
FAPESP: 07-59488-1
NIH/NIDCR: R01DE016132
NIH/NIDCR: R03TW007665
Date 2011-07-01
Published in Artificial Organs. Malden: Wiley-Blackwell, v. 35, n. 7, p. E129-E135, 2011.
ISSN 0160-564X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent E129-E135
Access rights Closed access
Type Article
Web of Science ID WOS:000292651100002

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