The role of the TP53 gene during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide

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dc.contributor.author Minicucci, E. M.
dc.contributor.author Ribeiro, D. A. [UNIFESP]
dc.contributor.author Silva, G. N. da
dc.contributor.author Pardini, M. I. M. C.
dc.contributor.author Montovani, J. C.
dc.contributor.author Salvadori, D. M. F.
dc.date.accessioned 2016-01-24T14:16:58Z
dc.date.available 2016-01-24T14:16:58Z
dc.date.issued 2011-07-01
dc.identifier http://dx.doi.org/10.1016/j.etp.2010.03.009
dc.identifier.citation Experimental and Toxicologic Pathology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 63, n. 5, p. 483-489, 2011.
dc.identifier.issn 0940-2993
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33855
dc.description.abstract The medium-term tongue carcinogenesis assay is a useful model for studying oral squamous cell carcinomas phase by phase. the aim of the present study was to investigate the expression of p53 by immunohistochemistry and examine the DNA sequence of exons 5, 6, 7, and 8 of Tp53 for mutations during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). A total of 30 male Wistar rats were treated with 4-nitroquinoline 1-oxide in their drinking water for 4, 12, and 20 weeks at a dose of 50 ppm. Ten animals were used as negative controls. No histopathological changes in the tongue epithelia were observed in the control group or in the treatment group after 4 weeks of 4NQO. Following 12 weeks of treatment, hyperplasia as well as epithelial dysplasia was found in both mild and moderate forms. At 20 weeks, moderate and/or severe oral dysplasia and squamous cell carcinoma of the tongue were found, and the majority of animals had squamous cell carcinoma. the levels of p53 protein were increased (p < 0.05) in pre-neoplastic lesions and in squamous cell carcinomas in some of the tumor cells in squamous cell carcinomas. No mutations were found in any of the exons that were evaluated after the 4-, 12-, or 20-week treatments. Taken together, our results suggest that p53 expression may be an important event in the malignant conversion, whereas Tp53 mutations are not involved in the multi-step tongue carcinogenesis of Wistar rats induced by 4NQO. (C) 2010 Elsevier GmbH. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 483-489
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Experimental and Toxicologic Pathology
dc.rights Acesso restrito
dc.subject Tp53 en
dc.subject Rat tongue mucosa en
dc.subject Oral squamous cell carcinoma en
dc.subject 4-Nitroquinoline 1-oxide en
dc.subject Direct DNA-sequencing en
dc.title The role of the TP53 gene during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution São Paulo State Univ
dc.description.affiliation Universidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.affiliation São Paulo State Univ, UNESP, Dept Dermatol & Radiotherapy, São Paulo, SP, Brazil
dc.description.affiliation São Paulo State Univ, UNESP, Dept Pathol, São Paulo, SP, Brazil
dc.description.affiliation São Paulo State Univ, UNESP, Dept Clin Med, São Paulo, SP, Brazil
dc.description.affiliation São Paulo State Univ, UNESP, Dept Ophthalmol & Otorhinolaryngol, São Paulo, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.sponsorshipID FAPESP: 05/59156-3
dc.description.sponsorshipID FAPESP: 07/01228-4
dc.description.sponsorshipID CNPq: 561357/2008-0
dc.identifier.doi 10.1016/j.etp.2010.03.009
dc.description.source Web of Science
dc.identifier.wos WOS:000291706600011



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