The lipid composition of a cell membrane modulates the interaction of an antiparasitic peptide at the air-water interface

The lipid composition of a cell membrane modulates the interaction of an antiparasitic peptide at the air-water interface

Author Herculano, Rondinelli Donizetti Google Scholar
Pavinatto, Felippe José Google Scholar
Caseli, Luciano Autor UNIFESP Google Scholar
D'Silva, Claudius Google Scholar
Oliveira, Osvaldo N Google Scholar
Institution Univ Estadual Paulista
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Manchester Metropolitan Univ
Abstract The antiparasitic property of peptides is believed to be associated with their interactions with the protozoan membrane, which calls for research on the identification of membrane sites capable of peptide binding. in this study we investigated the interaction of a lipophilicglutathioine peptide known to be effective against the African Sleeping Sickness (ASS - African Trypanosomiasis) and cell membrane models represented by Langmuir monolayers. It is shown that even small amounts of the peptide affect the monolayers of some phospholipids and other lipids, which points to a significant interaction. the latter did not depend on the electrical charge of the monolayer-forming molecules but the peptide action was particularly distinctive for cholesterol + sphingomyelin monolayers that roughly resemble rafts on a cell membrane. Using in situ polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), we found that the orientation of the peptide is affected by the phospholipids and dioctadecyldimethylammonium bromide (DODAB), but not in monolayers comprising cholesterol + sphingomyelin. in this mixed monolayer resembling rafts, the peptide still interacts and has some induced order, probably because the peptide molecules are fitted together into a compact monolayer. Therefore, the lipid composition of the monolayer modulates the interaction with the lipophilic glutathioine peptide, and this may have important implications in understanding how the peptide acts on specific sites of the protozoan membrane. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Cell membrane model
Langmuir monolayer
Raft
Lipophilic peptide
African Sleeping Sickness
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
rede nBioNet (Brazil)
Date 2011-07-01
Published in Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1808, n. 7, p. 1907-1912, 2011.
ISSN 0005-2736 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1907-1912
Origin http://dx.doi.org/10.1016/j.bbamem.2011.03.012
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000291172100016
URI http://repositorio.unifesp.br/handle/11600/33810

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