Jaw and long bone marrow derived osteoclasts differ in shape and their response to bone and dentin

Jaw and long bone marrow derived osteoclasts differ in shape and their response to bone and dentin

Author Azari, Azin Google Scholar
Schoenmaker, Ton Google Scholar
Souza Faloni, Ana Paula de Autor UNIFESP Google Scholar
Everts, Vincent Google Scholar
Vries, Teun J. de Google Scholar
Institution Univ Amsterdam
Universidade Federal de São Paulo (UNIFESP)
Abstract Increasing evidence suggests the existence of osteoclast diversity. Here we investigated whether precursors obtained from marrow of the mandibula or long bone could give rise to phenotypically different osteoclasts. Formation of multinucleated cells was assessed after culturing mouse marrow cells of the two bone types with macrophage colony stimulating factor (M-CSF) and receptor activator of NF kappa B ligand (RANKL) for up to 10 days on plastic, bone or dentin. Two times more osteoclasts formed from long bone marrow cells on bone compared to dentin, whereas higher numbers of jaw osteoclasts formed on dentin. Resorption of dentin or bone was similar for osteaclasts formed from both types of precursors. in contrast to jaw marrow derived osteoclasts, long bone osteoclasts predominantly had a multi-compartmented shape, with at least two nuclei containing compartments per cell. Osteoclasts on bone contained two times more actin rings than osteoclasts on dentin, regardless of their precursor origin. However, the area per osteoclast covered by actin rings was similar (20%) for both substrates. This study suggests that marrow cells obtained from different bones give rise to different osteoclasts. the substrate on which the osteoclasts are generated plays a role in steering their formation rather than their resorption. (C) 2011 Elsevier Inc. All rights reserved.
Keywords Osteoclast diversity
Jaw
Long bone
Bone marrow
Mineralized tissues
Language English
Date 2011-06-03
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 409, n. 2, p. 205-210, 2011.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 205-210
Origin http://dx.doi.org/10.1016/j.bbrc.2011.04.120
Access rights Closed access
Type Article
Web of Science ID WOS:000291779100010
URI http://repositorio.unifesp.br/handle/11600/33797

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