Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice

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dc.contributor.author Aquino, Ivani
dc.contributor.author Perazzo, Fábio Ferreira [UNIFESP]
dc.contributor.author Maistro, Edson Luis
dc.date.accessioned 2016-01-24T14:16:46Z
dc.date.available 2016-01-24T14:16:46Z
dc.date.issued 2011-06-01
dc.identifier http://dx.doi.org/10.1016/j.fct.2011.03.016
dc.identifier.citation Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 49, n. 6, p. 1335-1339, 2011.
dc.identifier.issn 0278-6915
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33716
dc.description.abstract Artesunate is a derivate of artemisinin that is both an antimalarial agent and acts cytotoxically on tumor cells. Despite its therapeutic use, its in vivo genotoxic potential has still not been evaluated. This study, therefore, was an investigation into the effects of a single oral administration of artesunate with an in vivo comet assay that analyzed leukocytes from peripheral blood and liver cells, and a micronucleus (MN) assay of bone marrow cells from male Swiss mice. the artesunate was administered by oral gavage at doses of 5, 50 and 100 mg/kg. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). the results demonstrate that artesunate induced significant DNA damage only in liver cells and that high doses of artesunate caused an increase in the mean number of micronucleated polychromatic erythrocytes (MNPCE). Under our experimental conditions, artesunate showed weak genotoxic effects at low doses and clastogenic effects at high doses. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution about either continuous or high-dose use of artesunate by humans. (C) 2011 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 1335-1339
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Food and Chemical Toxicology
dc.rights Acesso aberto
dc.subject Artesunate en
dc.subject Artemisinin derivate en
dc.subject Micronucleus test comet assay en
dc.subject Clastogenicity en
dc.title Genotoxicity assessment of the antimalarial compound artesunate in somatic cells of mice en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Estadual Paulista
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Estadual Paulista, UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP, Brazil
dc.description.affiliation Univ Estadual Paulista, UNESP, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, Brazil
dc.description.sponsorshipID CNPq: 306544/2006-7
dc.description.sponsorshipID FAPESP: 2008/51175-7
dc.identifier.file WOS000291514800021.pdf
dc.identifier.doi 10.1016/j.fct.2011.03.016
dc.description.source Web of Science
dc.identifier.wos WOS:000291514800021



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