Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation

Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation

Autor Visniauskas, Bruna Autor UNIFESP Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Carmona, Adriana K. Autor UNIFESP Google Scholar
D'Almeida, Vania Autor UNIFESP Google Scholar
Melo, Robson L. de Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Chagas, Jair R. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Butantan Inst
Resumo Proteases are essential either for the release of neuropeptides from active or inactive proteins or for their inactivation. Neuropeptides have a fundamental role in sleep-wake cycle regulation and their actions are also likely to be regulated by proteolytic processing. Using fluorescence resonance energy transfer substrates, specific protease inhibitors and real-time PCR we demonstrate changes in angiotensin I-converting enzyme (ACE) expression and proteolytic activity in the central nervous system in an animal model of paradoxical sleep deprivation during 96 h (PSD). Male rats were distributed into five groups (PSD, 24 h, 48 h and 96 h of sleep recovery after PSD and control). ACE activity and mRNA levels were measured in hypothalamus, hippocampus, brainstem, cerebral cortex and striatum tissue extracts. in the hypothalamus, the significant decrease in activity and mRNA levels, after PSD, was only totally reversed after 96 h of sleep recovery. in the brainstem and hippocampus, although significant, changes in mRNA do not parallel changes in ACE specific activity. Changes in ACE activity could affect angiotensin II generation, angiotensin 1-7, bradykinin and opioid peptides metabolism. ACE expression and activity modifications are likely related to some of the physiological changes (cardiovascular, stress, cognition, metabolism function, water and energy balance) observed during and after sleep deprivation.
Assunto ACE
angiotensin II
central nervous system
hypothalamus
paradoxical sleep deprivation
protease
Idioma Inglês
Financiador Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento FAPESP: 98/14303-3
CNPq: 481104/2004-6
Data 2011-04-01
Publicado em Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 392, n. 6, p. 547-553, 2011.
ISSN 1431-6730 (Sherpa/Romeo, fator de impacto)
Editor Walter de Gruyter & Co
Extensão 547-553
Fonte http://dx.doi.org/10.1515/BC.2011.057
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000290715200007
URI http://repositorio.unifesp.br/handle/11600/33627

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