The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines

The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines

Author Nakahata, Adriana Miti Autor UNIFESP Google Scholar
Mayer, Barbara Google Scholar
Ries, Christian Google Scholar
Andrade de Paula, Claudia Alessandra Autor UNIFESP Google Scholar
Karow, Marisa Google Scholar
Neth, Peter Google Scholar
Sampaio, Misako U. Autor UNIFESP Google Scholar
Jochum, Marianne Google Scholar
Oliva, Maria Luiza V. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Munich
Abstract Supplementary to the efficient inhibition of trypsin, chymotrypsin, plasma kallikrein, and plasmin already described by the EcTI inhibitor from Enterolobium contortisiliquum, it also blocks human neutrophil elastase (K(iapp)=4.3 nM) and prevents phorbol ester (PMA)-stimulated activation of matrix metalloproteinase (MMP)-2 probably via interference with membrane-type 1 (MT1)-MMP. Moreover, plasminogen-induced activation of proMMP-9 and processing of active MMP-2 was also inhibited. Furthermore, the effect of EcTI on the human cancer cell lines HCT116 and HT29 (colorectal), SkBr-3 and MCF-7 (breast), K562 and THP-1 (leukemia), as well as on human primary fibroblasts and human mesenchymal stem cells (hMSCs) was studied. EcTI inhibited in a concentration range of 1.0-2.5 mu M rather specifically tumor cell viability without targeting primary fibroblasts and hMSCs. Taken together, our data indicate that the polyspecific proteinase inhibitor EcTI prevents proMMP activation and is cytotoxic against tumor cells without affecting normal tissue remodeling fibroblasts or regenerative hMSCs being an important tool in the studies of tumor cell development and dissemination.
Keywords cancer
Kunitz inhibitors
matrix metalloproteinases
mesenchymal stem cells
plant proteinases
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-04-01
Published in Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 392, n. 4, p. 327-336, 2011.
ISSN 1431-6730 (Sherpa/Romeo, impact factor)
Publisher Walter de Gruyter & Co
Extent 327-336
Origin http://dx.doi.org/10.1515/BC.2010.023
Access rights Closed access
Type Article
Web of Science ID WOS:000288317600007
URI http://repositorio.unifesp.br/handle/11600/33626

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