In vivo infection by Trypanosoma cruzi: the conserved FLY domain of the gp85/trans-sialidase family potentiates host infection

In vivo infection by Trypanosoma cruzi: the conserved FLY domain of the gp85/trans-sialidase family potentiates host infection

Author Tonelli, Renata Rosito Autor UNIFESP Google Scholar
Torrecilhas, Ana Claudia Trocoli Google Scholar
Jacysyn, J. F. Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Colli, Walter Google Scholar
Alves, Maria Julia Manso Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Trypanosoma cruzi is a protozoan parasite that infects vertebrates, causing in humans a pathological condition known as Chagas' disease. the infection of host cells by T. cruzi involves a vast collection of molecules, including a family of 85 kDa GPI-anchored glycoproteins belonging to the gp85/trans-sialidase superfamily, which contains a conserved cell-binding sequence (VTVXNVFLYNR) known as FLY, for short. Herein, it is shown that BALB/c mice administered with a single dose (1 mu g/animal, intraperitoneally) of FLY-synthetic peptide are more susceptible to infection by T. cruzi, with increased systemic parasitaemia (2-fold) and mortality. Higher tissue parasitism was observed in bladder (7.6-fold), heart (3-fold) and small intestine (3.6-fold). Moreover, an intense inflammatory response and increment of CD4(+) T cells (1.7-fold) were detected in the heart of FLY-primed and infected animals, with a 5-fold relative increase of CD4(+)CD25(+)FoxP3(+) T (Treg) cells. Mice treated with anti-CD25 antibodies prior to infection, showed a decrease in parasitaemia in the FLY model employed. in conclusion, the results suggest that FLY facilitates in vivo infection by T. cruzi and concurs with other factors to improve parasite survival to such an extent that might influence the progression of pathology in Chagas' disease.
Keywords Trypanosoma cruzi
Chagas' disease
CD4(+)CD25(+)FoxP3(+) T cells
gp85/trans-sialidase glycoprotein family
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2004/03303-5
Date 2011-04-01
Published in Parasitology. New York: Cambridge Univ Press, v. 138, n. 4, p. 481-492, 2011.
ISSN 0031-1820 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 481-492
Access rights Closed access
Type Article
Web of Science ID WOS:000288614600007

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