Biofilm production and evaluation of antifungal susceptibility amongst clinical Candida spp. isolates, including strains of the Candida parapsilosis complex

Biofilm production and evaluation of antifungal susceptibility amongst clinical Candida spp. isolates, including strains of the Candida parapsilosis complex

Author Melo, Analy Salles de Azevedo Autor UNIFESP Google Scholar
Bizerra, Fernando Cesar Autor UNIFESP Google Scholar
Freymüller-Haapalainen, Edna Autor UNIFESP Google Scholar
Arthington-Skaggs, Beth A. Google Scholar
Colombo, Arnaldo Lopes Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Ctr Dis Control & Prevent
Abstract Candida cells can form biofilms that frequently are sources of infections and are less susceptible to antifungal drugs. Some authors have reported that Candida orthopsilosis and Candida metapsilosis isolates are not able to produce biofilms in vitro and there are no studies available on biofilm susceptibility for these species to antifungals. the aims of this study were to (i) quantify Candida spp. biofilms in vitro, and (ii) test the in vitro susceptibilities of Candida spp. biofilms to fluconazole (FLC) and amphotericin B (AMB). Isolates studied included four Candida albicans, six C. tropicalis, seven C. parapsilosis, eight C. orthopsilosis, and five C. metapsilosis. We compared two different methods to evaluate biofilm production, i. e., crystal violet (CV) staining and XTT-reduction assays (XTT). Scanning electron microscopy (SEM) was used to observe high, medium and low biofilm producing isolates screened by these two methods. To determine the minimum biofilm eradication concentration (MBEC) for FLC and AMB, XTT-reduction assay was used to measure cell metabolic activity. Biofilm quantification by CV and XTT showed that C. tropicalis isolates were the highest biofilm producer, followed by C. albicans, C. parapsilosis, C. orthopsilosis and C. metapsilosis. Examination of SEM images revealed that the extent of biofilms formed by high, medium, and low producers was highly correlated to the results generated by CV assay. Biofilm of all the isolates evaluated were resistant to FLC (MBEC(80) >= 256 ug/ml) but, in general, susceptible to AMB, except for six C. parapsilosis strains (MBEC (80) >= 8 ug/ml).
Keywords Candida spp.
biofim production and susceptibility
Candida orthopsilosis
Candida metapsilosis
Language English
Sponsor CDC (Centers for Disease Control and Prevention, USA)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2005/50778-1
CNPq: 304802/2007-7
Date 2011-04-01
Published in Medical Mycology. London: Informa Healthcare, v. 49, n. 3, p. 253-262, 2011.
ISSN 1369-3786 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 253-262
Origin http://dx.doi.org/10.3109/13693786.2010.530032
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288323400004
URI http://repositorio.unifesp.br/handle/11600/33581

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