A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts

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dc.contributor.author Kioshima, Érika Seki [UNIFESP]
dc.contributor.author Aliperti, Fabiana [UNIFESP]
dc.contributor.author Maricato, Juliana Terzi [UNIFESP]
dc.contributor.author Mortara, Renato Arruda [UNIFESP]
dc.contributor.author Bagagli, Eduardo
dc.contributor.author Mariano, Mario [UNIFESP]
dc.contributor.author Lopes, Jose Daniel [UNIFESP]
dc.date.accessioned 2016-01-24T14:06:13Z
dc.date.available 2016-01-24T14:06:13Z
dc.date.issued 2011-03-01
dc.identifier http://dx.doi.org/10.1016/j.micinf.2010.10.019
dc.identifier.citation Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 3, p. 251-260, 2011.
dc.identifier.issn 1286-4579
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33484
dc.description.abstract This work was conducted to identify virulence biomarkers for Paracoccidioides brasiliensis (Pb), the fungus responsible for Paracoccidioidomycosis (PCM), a systemic disease endemic in Latin America. Measurement of mortality showed that all B10.A mice were killed after 250 days by the virulent Pb18 isolate while only one of the mice that received the attenuated counterpart died. Also, number of lung CFUs from virulent Pb18 inoculated mice were much higher when these isolates were compared. Phage display methodology allowed selection of three phages that specifically bound to virulent Pb18. Variability of p04 phage binding to different Pb isolates were examples of variability of expression by the fungus of its binding molecule, strongly suggesting p04 as a biomarker of virulence. in vitro, its derived peptide pep04 killed only virulent fungi, and confocal microscopy showed that it was internalized only by the virulent isolate. Pep04 blocked establishment of Pb infection in mice and virulent Pb18 pre-incubated with p04 showed significantly inhibited lung infection. Furthermore, infected mice treated with p04 showed highly significant reduction in lung CFUs. These findings firmly establish p04 as a biomarker of Pb virulence. Therefore, after proper peptide engineering, p04 may become a useful adjuvant for the distressing treatment of PCM. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 251-260
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Microbes and Infection
dc.rights Acesso aberto
dc.subject P. brasiliensis en
dc.subject Virulence en
dc.subject Biomarkers en
dc.title A synthetic peptide selectively kills only virulent Paracoccidioides brasiliensis yeasts en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Univ Estadual Paulista
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliation State Univ São Paulo, Dept Microbiol, Inst Biosci Botucatu, Botucatu, SP, Brazil
dc.description.affiliation Univ Estadual Paulista, Inst Hlth, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 04/04471-9
dc.identifier.file WOS000287615000006.pdf
dc.identifier.doi 10.1016/j.micinf.2010.10.019
dc.description.source Web of Science
dc.identifier.wos WOS:000287615000006


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