Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil

Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil

Author Cergole-Novella, Maria Cecilia Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Castanheira, Mariana Autor UNIFESP Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
JMI Labs
Abstract Antimicrobial resistance patterns and molecular characteristics were determined in thirty-two Shiga-toxin-producing Escherichia coli (STEC) strains previously identified in São Paulo State associated with human infections (n = 21) and in cattle feces (n = 11). the highest resistance rates were identified for tetracycline (100%), streptomycin (78%) and trimethoprim sulfamethoxazole (56%). Eleven STEC strains showed resistance to ampicillin and carried bla(TEM) that was confirmed as bla(TEM-1) in one representative isolate. the class 1 integrase gene (intI1) was detected in seven (22%) strains, and most of them belonged to the O111:H8 serotype. the class 1 integron was located on plasmids in five of the seven STEC strains, and conjugation assays confirmed the plasmid support of those resistant determinants. STEC strains were genetically classified into the B I group, and PFGE analysis showed that most of the strains in each serogroup were grouped into the same cluster (80-97% similarity). the presence of a class 1 integron and bla(TEM-1) genes is described for the first time among STEC isolates in Brazil and clearly represents a public health concern. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Keywords Shiga-toxin-producing Escherichia coli
Resistance genes
Class 1 integron
Phylogenetic background
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-02-01
Published in Research in Microbiology. Amsterdam: Elsevier B.V., v. 162, n. 2, p. 117-123, 2011.
ISSN 0923-2508 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 117-123
Origin http://dx.doi.org/10.1016/j.resmic.2010.09.022
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288880500003
URI http://repositorio.unifesp.br/handle/11600/33385

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