Neuroprotective effect of pyruvate and oxaloacetate during pilocarpine induced status epilepticus in rats

Neuroprotective effect of pyruvate and oxaloacetate during pilocarpine induced status epilepticus in rats

Author Carvalho, Andrezza Sossai Rodrigues Autor UNIFESP Google Scholar
Torres, Laila Brito Autor UNIFESP Google Scholar
Persike, Daniele Suzete Autor UNIFESP Google Scholar
Fernandes, Maria Jose da Silva Autor UNIFESP Google Scholar
Amado, Débora Autor UNIFESP Google Scholar
Naffah-Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Silva, Alexandre Valotta da Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Recent research data have shown that systemic administration of pyruvate and oxaloacetate causes an increased brain-to-blood glutamate efflux. Since increased release of glutamate during epileptic seizures can lead to excitotoxicity and neuronal cell death, we tested the hypothesis that glutamate scavenging mediated by pyruvate and oxaloacetate systemic administration could have a neuroprotective effect in rats subjected to status epilepticus (SE). SE was induced by a single dose of pilocarpine (350 mg/kg i.p.). Thirty minutes after SE onset, a single dose of pyruvate (250 mg/kg i.p.), oxaloacetate (1.4 mg/kg i.p.), or both substances was administrated. Acute neuronal loss in hippocampal regions CA1 and hilus was quantitatively determined five hours after SE onset, using the optical fractionator method for stereological cell counting. Apoptotic cascade in the hippocampus was also investigated seven days after SE using caspase-1 and -3 activity assays. SE-induced neuronal loss in CA1 was completely prevented in rats treated with pyruvate plus oxaloacetate. the SE-induced caspase-1 activation was significantly reduced when rats were treated with oxaloacetate or pyruvate plus oxaloacetate. the treatment with pyruvate and oxaloacetate caused a neuroprotective effect in rats subjected to pilocarpine-induced SE. (C) 2010 Elsevier B.V. All rights reserved.
Keywords Brain
Hippocampus
Stereology
Epilepsy
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-02-01
Published in Neurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 58, n. 3, p. 385-390, 2011.
ISSN 0197-0186 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 385-390
Origin http://dx.doi.org/10.1016/j.neuint.2010.12.014
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000295749500015
URI http://repositorio.unifesp.br/handle/11600/33383

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