Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigens

Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigens

Autor Fernandes, Geisa Ferreira Autor UNIFESP Google Scholar
Lopes-Bezerra, Leila Maria Google Scholar
Bernardes-Engemann, Andrea Reis Google Scholar
Pacheco Schubach, Tania Maria Google Scholar
Galvao Dias, Maria Adelaide Google Scholar
Pereira, Sandro Antonio Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade do Estado do Rio de Janeiro (UERJ)
Fiocruz MS
Ctr Zoonosis Prefeitura São Paulo
Resumo The main objective of this study is to standardize an ELISA for the diagnosis of feline sporotrichosis. Sporothrix schenckii is the etiological agent of human and animal sporotrichosis. Cats may act as reservoirs for S. schenckii and can transmit the infection to humans by a bite or scratch. There are few methods for the serological diagnosis of fungal diseases in animals. in this paper, an ELISA test for the diagnosis of cat sporotrichosis is proposed, which detects S. schenckii-specific antibodies in feline sera. Two different kinds of antigens were used: SsCBF, a specific molecule from S. schenckii that consists of a Con A-binding fraction derived from a peptido-rhamnomannan component of the cell wall, and a S. schenckii crude exoantigen preparation. the ELISA was developed, optimized, and evaluated using sera from 30 cats with proven sporotrichosis (by culture isolation); 22 sera from healthy feral cats from a zoonosis center were used as negative controls. SsCBF showed 90% sensitivity and 96% specificity in ELISA; while crude exoantigens demonstrated 96% sensitivity and 98% specificity. the ELISA assay described here would be a valuable screening tool for the detection of specifics. schenckii antibodies in cats with sporotrichosis. the assay is inexpensive, quick to perform, easy to interpret, and permits the diagnosis of feline sporotrichosis. (C) 2010 Elsevier B.V. All rights reserved.
Assunto Sporothrix schenckii
Sporotrichosis
ELISA
SsCBF antigen
Crude exoantigens
Feline
Cat
Idioma Inglês
Data 2011-01-27
Publicado em Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 147, n. 3-4, p. 445-449, 2011.
ISSN 0378-1135 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 445-449
Fonte http://dx.doi.org/10.1016/j.vetmic.2010.07.007
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000287119100030
URI http://repositorio.unifesp.br/handle/11600/33363

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