A Trypsin Inhibitor from Sapindus saponaria L. Seeds: Purification, Characterization, and Activity Towards Pest Insect Digestive Enzyme

A Trypsin Inhibitor from Sapindus saponaria L. Seeds: Purification, Characterization, and Activity Towards Pest Insect Digestive Enzyme

Author Macedo, Maria Ligia R. Google Scholar
Diz Filho, Eduardo B. S. Google Scholar
Freire, Mariadas Gracas M. Google Scholar
Oliva, Maria Luiza V. Autor UNIFESP Google Scholar
Sumikawa, Joana T. Autor UNIFESP Google Scholar
Toyama, Marcos H. Google Scholar
Marangoni, Sergio Google Scholar
Institution Universidade Federal de Mato Grosso do Sul (UFMS)
Universidade Estadual de Campinas (UNICAMP)
Inst Super CENSA
Universidade Federal de São Paulo (UNIFESP)
Abstract The present paper describes the purification, characterization and determination of the partial primary structure of the first trypsin inhibitor isolated from the family Sapindaceae. A highly stable, potent trypsin inhibitor (SSTI) was purified to homogeneity. SDS-PAGE analysis revealed that the protein consists of a two-polypeptide chain with molecular masses of approximately 15 and 3 kDa. the purified inhibitor inhibited bovine trypsin at a 1:1 M ratio. Kinetic analysis revealed that the protein is a competitive inhibitor with an equilibrium dissociation constant of 10(-9) M for trypsin. the partial NH(2)- terminal sequence of 36 amino acids in SSTI indicates homology with other members of the trypsin-inhibitor family from different sources. This inhibitor is highly stable in the presence of denaturing agents. SSTI showed significant inhibitory activity against trypsin-like proteases present in the larval midgut on Anagasta kuehniella, Corcyra cephalonica, Diatreae saccharalis and Anticarsia gemmatalis.
Keywords Trypsin inhibitor
Sapindaceae
Lepidopteran
Kinetic studies
Language English
Sponsor FUNDECT(Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
PROPP/UFMS (Pro-Reitoria de Pesquisa e Pos-graduacao da Universidade Federal de Mato Grosso do Sul)
Date 2011-01-01
Published in Protein Journal. New York: Springer, v. 30, n. 1, p. 9-19, 2011.
ISSN 1572-3887 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 9-19
Origin http://dx.doi.org/10.1007/s10930-010-9296-7
Access rights Closed access
Type Article
Web of Science ID WOS:000286464400002
URI http://repositorio.unifesp.br/handle/11600/33265

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