Inhibitory Activity of alpha-Tocoferol on Apoptosis in the Rat Bladder Wall Subjected to Androgen Deprivation

Inhibitory Activity of alpha-Tocoferol on Apoptosis in the Rat Bladder Wall Subjected to Androgen Deprivation

Author Ludwig, Luana Google Scholar
Seraphim, Deborah Google Scholar
Lorenzetti, Fabio Autor UNIFESP Google Scholar
Bertolla, Ricardo Pimenta Google Scholar
Ortiz, Valdemar Google Scholar
Dambros, Miriam Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Alpha-tocopherol (2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-chromon-8-ol) is used in many previous urological studies. Thus to add to this knowledge in this study we studied the potential inhibitory activity on oxidative stress and process apoptosis on bladder wall in male rats subjected to androgen deprivation. A causal relationship between lower testosterone levels and apoptosis, as a component of castration-induced muscle atrophy, has been shown. Male Wistar rats (250-300 g) were used in this experiment, divided into four groups: control (sham operation; n=10); castration (n=10); castration, with alpha-tocopherol supplementation over preceding four weeks (n=10); and castration, with alpha-tocopherol supplementation over preceding four weeks and subsequent eight weeks (n=10). Activated caspase-3 was detected using a previously described technique, with analysis using stereological methodology. Nonparametric methods were used to test statistical significance, taking a significance level of P < 0.005. This study was approved by the ethics committee of the university where the project was developed. the serum testosterone concentrations before castration were less than 20 pg/ml. Analysis of 8-isoprostane showed statistical significance (P < 0.0003). the volumetric density of caspase-3 showed significant differences between the groups. There was no statistical significance regarding caspase-3 between sham and alpha-tocopherol plus castration or between the groups that received alpha-tocopherol supplementation. the observations showed that there was greater apoptosis in the group with castration alone than in the groups with alpha-tocopherol supplementation. This finding, together with the induced androgen deprivation and higher 8-isoprostane levels, corroborates the hypothesis that alpha-tocopherol supplementation has an important protective effect under conditions of oxidative stress, thereby avoiding the apoptotic process, especially regarding aging. Neurourol. Urodynam. 30: 194-198, 2011. (C) 2010 Wiley-Liss, Inc.
Keywords alpha-tocoferol
androgen deprivation
apoptosis
oxidative stress
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 07/50154-3
Date 2011-01-01
Published in Neurourology and Urodynamics. Malden: Wiley-Blackwell, v. 30, n. 1, p. 194-198, 2011.
ISSN 0733-2467 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 194-198
Origin http://dx.doi.org/10.1002/nau.20857
Access rights Closed access
Type Article
Web of Science ID WOS:000285980300034
URI http://repositorio.unifesp.br/handle/11600/33253

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