In Vivo Treatments with Fulvestrant and Anastrozole Differentially Affect Gene Expression in the Rat Efferent Ductules

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dc.contributor.author Oliveira Gomes, Gisele Renata [UNIFESP]
dc.contributor.author Yasuhara, Fabiana [UNIFESP]
dc.contributor.author Siu, Erica Rosanna [UNIFESP]
dc.contributor.author Ferreira Fernandes, Sheilla Alessandra [UNIFESP]
dc.contributor.author Werneck Avellar, Maria Christina [UNIFESP]
dc.contributor.author Magalhaes Lazari, Maria Fatima [UNIFESP]
dc.contributor.author Porto, Catarina Segreti [UNIFESP]
dc.date.accessioned 2016-01-24T14:05:51Z
dc.date.available 2016-01-24T14:05:51Z
dc.date.issued 2011-01-01
dc.identifier http://dx.doi.org/10.1095/biolreprod.110.085340
dc.identifier.citation Biology of Reproduction. Madison: Soc Study Reproduction, v. 84, n. 1, p. 52-61, 2011.
dc.identifier.issn 0006-3363
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33193
dc.description.abstract Estrogen plays a key role in maintaining the morphology and function of the efferent ductules We previously demonstrated that the antiestrogen fulvestrant markedly affected gene expression in the rat efferent ductules the mechanism of fulvestrant action to modulate gene expression may involve not only the blockade of ESR1 and ESR2 estrogen receptors, but also the activation of ESR1 and ESR2 when the receptors are tethered to AP 1 or SP1 transcription factors, or the activation of the G protein-coupled estrogen receptor 1 We therefore compared the effects of two strategies to interfere with estrogen action in the rat efferent ductules treatment with fulvestrant or with the aromatase inhibitor anastrozole Whereas fulvestrant markedly increased Mmp7 and Spp1, and reduced Npfx1 mRNA levels, no changes were observed with anastrozole Fulvestrant caused changes in epithelial morphology that were not seen with anastrozole Fulvestrant shifted MMP7 immunolocalization in the epithelial cells from the supranuclear to the apical region, this effect was less pronounced with anastrozole in vitro studies of S-35-methiomne incorporation showed that protein release was increased, whereas tissue protein content in the efferent ductules of fulvestrant-treated rats was decreased Although fulvestrant markedly affected gene expression, no changes were observed on AP 1 and SP1 DNA-binding activity the blockade of ESRs seems to be the major reason explaining the differences between both treatments At least some of the effects of fulvestrant appear to result from compensatory mechanisms activated by the dramatic changes caused by ESR1 blockade en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 52-61
dc.language.iso eng
dc.publisher Soc Study Reproduction
dc.relation.ispartof Biology of Reproduction
dc.rights Acesso aberto
dc.subject estradiol receptor en
dc.subject gene regulation en
dc.subject male reproductive tract en
dc.title In Vivo Treatments with Fulvestrant and Anastrozole Differentially Affect Gene Expression in the Rat Efferent Ductules en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Inst Nacl Farmacol & Biol Mol
dc.description.affiliation Universidade Federal de São Paulo, Sect Expt Endocrinol, Dept Pharmacol, INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliation Inst Nacl Farmacol & Biol Mol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Sect Expt Endocrinol, Dept Pharmacol, INFAR, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2008/56564 1
dc.identifier.doi 10.1095/biolreprod.110.085340
dc.description.source Web of Science
dc.identifier.wos WOS:000285658600009



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