Detection of Clonal Immunoglobulin and T-Cell Receptor Gene Rearrangements in Childhood Acute Lymphoblastic Leukemia Using a Low-Cost PCR Strategy

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dc.contributor.author Assumpcao, Juliana Godoy
dc.contributor.author Ganazza, Monica Aparecida
dc.contributor.author Araujo, Marcela de
dc.contributor.author Silva, Ariosto Siqueira
dc.contributor.author Scrideli, Carlos Alberto
dc.contributor.author Brandalise, Silvia Regina
dc.contributor.author Yunes, Jose Andres
dc.date.accessioned 2016-01-24T14:05:49Z
dc.date.available 2016-01-24T14:05:49Z
dc.date.issued 2010-12-15
dc.identifier http://dx.doi.org/10.1002/pbc.22709
dc.identifier.citation Pediatric Blood & Cancer. Hoboken: Wiley-Blackwell, v. 55, n. 7, p. 1278-1286, 2010.
dc.identifier.issn 1545-5009
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/33171
dc.description.abstract Background Imunoglobulin (Ig) and T cell receptor (TCR) gene rearrangements function as specific markers for minimal residual disease (MRD) which is one of the best predictors of outcome in childhood acute lymphoblastic leukemia (ALL) We recently reported on the prognostic value of MRD during the induction of remission through a simplified PCR method Here we report on gene rearrangement frequencies and offer guidelines for the application of the technique Procedure Two hundred thirty three children had DNA extracted from bone marrow Ig and TCR gene rearrangements were amplified using consensus primers and conventional PCR PCR products were submitted to homo/heteroduplex analysis A computer program was designed to define combinations of targets for clonal detection using a minimum set of primers and reactions Results At least one clonal marker could be detected in 98% of the patients and two markers in approximately 80% the most commonly rear ringed genes in precursor B cell ALL were IgH (75%) TCRD (59%) IgK (55%), and TCRG (54%) the most commonly rearranged genes for TALL were TCRG (100%) and TCRD (24%) the sensitivity of primers was limited to the detection of 1 leukemic cell among 100 normal cells Conclusions We propose that eight PCR reactions per ALL subtype would allow for the detection of two markers in most cases in addition these reactions ire suitable for MRD monitoring especially when aiming the selection of patients with high MRD levels (>= 10(-2)) at the end of induction therapy Such an approach would be very useful in centers with limited financial resources Pediatr Blood Cancer 2010 55 1278-1286 (C) 2010 Wiley Liss Inc en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 1278-1286
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Pediatric Blood & Cancer
dc.rights Acesso restrito
dc.subject acute lymphoblastic leukemia en
dc.subject immunoglobulin (Ig) en
dc.subject low income countries en
dc.subject minimal residual disease en
dc.subject T cell receptor (TCR) en
dc.title Detection of Clonal Immunoglobulin and T-Cell Receptor Gene Rearrangements in Childhood Acute Lymphoblastic Leukemia Using a Low-Cost PCR Strategy en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Ctr Infantil Boldrini
dc.contributor.institution H Lee Moffitt Canc Ctr & Res Inst
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Ctr Infantil Boldrini, Mol Biol Lab, BR-13083210 Campinas, SP, Brazil
dc.description.affiliation H Lee Moffitt Canc Ctr & Res Inst, Dept Integrat Math Oncol, Tampa, FL USA
dc.description.affiliation Univ São Paulo, Dept Pediat, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliation Univ Estadual Campinas, Dept Pediat, Campinas, SP, Brazil
dc.description.sponsorshipID FAPESP: 05/02279-6
dc.identifier.doi 10.1002/pbc.22709
dc.description.source Web of Science
dc.identifier.wos WOS:000284023600007



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