Detection of Clonal Immunoglobulin and T-Cell Receptor Gene Rearrangements in Childhood Acute Lymphoblastic Leukemia Using a Low-Cost PCR Strategy

Detection of Clonal Immunoglobulin and T-Cell Receptor Gene Rearrangements in Childhood Acute Lymphoblastic Leukemia Using a Low-Cost PCR Strategy

Author Assumpcao, Juliana Godoy Google Scholar
Ganazza, Monica Aparecida Google Scholar
Araujo, Marcela de Google Scholar
Silva, Ariosto Siqueira Google Scholar
Scrideli, Carlos Alberto Google Scholar
Brandalise, Silvia Regina Google Scholar
Yunes, Jose Andres Google Scholar
Institution Ctr Infantil Boldrini
H Lee Moffitt Canc Ctr & Res Inst
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background Imunoglobulin (Ig) and T cell receptor (TCR) gene rearrangements function as specific markers for minimal residual disease (MRD) which is one of the best predictors of outcome in childhood acute lymphoblastic leukemia (ALL) We recently reported on the prognostic value of MRD during the induction of remission through a simplified PCR method Here we report on gene rearrangement frequencies and offer guidelines for the application of the technique Procedure Two hundred thirty three children had DNA extracted from bone marrow Ig and TCR gene rearrangements were amplified using consensus primers and conventional PCR PCR products were submitted to homo/heteroduplex analysis A computer program was designed to define combinations of targets for clonal detection using a minimum set of primers and reactions Results At least one clonal marker could be detected in 98% of the patients and two markers in approximately 80% the most commonly rear ringed genes in precursor B cell ALL were IgH (75%) TCRD (59%) IgK (55%), and TCRG (54%) the most commonly rearranged genes for TALL were TCRG (100%) and TCRD (24%) the sensitivity of primers was limited to the detection of 1 leukemic cell among 100 normal cells Conclusions We propose that eight PCR reactions per ALL subtype would allow for the detection of two markers in most cases in addition these reactions ire suitable for MRD monitoring especially when aiming the selection of patients with high MRD levels (>= 10(-2)) at the end of induction therapy Such an approach would be very useful in centers with limited financial resources Pediatr Blood Cancer 2010 55 1278-1286 (C) 2010 Wiley Liss Inc
Keywords acute lymphoblastic leukemia
immunoglobulin (Ig)
low income countries
minimal residual disease
T cell receptor (TCR)
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 05/02279-6
Date 2010-12-15
Published in Pediatric Blood & Cancer. Hoboken: Wiley-Blackwell, v. 55, n. 7, p. 1278-1286, 2010.
ISSN 1545-5009 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1278-1286
Access rights Closed access
Type Article
Web of Science ID WOS:000284023600007

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