LOW-INTENSITY PULSED ULTRASOUND PRODUCED AN INCREASE of OSTEOGENIC GENES EXPRESSION DURING the PROCESS of BONE HEALING in RATS

LOW-INTENSITY PULSED ULTRASOUND PRODUCED AN INCREASE of OSTEOGENIC GENES EXPRESSION DURING the PROCESS of BONE HEALING in RATS

Author Favaro-Pipi, Elaine Google Scholar
Bossini, Paulo Google Scholar
Oliveira, Poliani de Google Scholar
Ribeiro, Juliana Uema Google Scholar
Tim, Carla Google Scholar
Parizotto, Nivaldo A. Google Scholar
Alves, Jose Marcos Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Selistre de Araujo, Heloisa Sobreiro Google Scholar
Muniz Renno, Ana Claudia Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de São Carlos (UFSCar)
Universidade de São Paulo (USP)
Abstract The aim of this study was to measure the temporal expression of osteogenic genes during the process of bone healing in low-intensity pulsed ultrasound (LIPUS) treated bone defects by means of histopathologic and real-time polymerase chain reaction (PCR) analysis. Animals were randomly distributed into two groups (n = 30): control group (bone defect without treatment) and LIPUS treated (bone defect treated with LIPUS). On days 7, 13 and 25 postinjury, 10 rats per group were sacrificed. Rats were treated with a 30 mW/cm(2) LIPUS. the results pointed out intense new bone formation surrounded by highly vascularized connective tissue presenting a slight osteogenic activity, with primary bone deposition was observed in the group exposed to LIPUS in the intermediary (13 days) and late stages of repair (25 days) in the treated animals. in addition, quantitative real-time polymerase chain reaction (RT-qPCR) showed an upregulation of bone morphogenetic protein 4 (BMP4), osteocalcin and Runx2 genes 7 days after the surgery. in the intermediary period, there was no increase in the expression. the expression of alkaline phosphatase, BMP4 and Runx2 was significantly increased at the last period. Our results indicate that LIPUS therapy improves bone repair in rats and upregulated osteogenic genes, mainly at the late stages of recovery. (E-mail: a.renno@unifesp.br) (C) 2010 Published by Elsevier Inc. on behalf of World Federation for Ultrasound in Medicine & Biology.
Keywords Bone repair
Rats
Low-intensity pulsed ultrasound
Osteogenesis
Genes
Quantitative PCR
Language English
Date 2010-12-01
Published in Ultrasound in Medicine and Biology. New York: Elsevier B.V., v. 36, n. 12, p. 2057-2064, 2010.
ISSN 0301-5629 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 2057-2064
Origin http://dx.doi.org/10.1016/j.ultrasmedbio.2010.07.012
Access rights Closed access
Type Article
Web of Science ID WOS:000284334400012
URI http://repositorio.unifesp.br/handle/11600/33138

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account