Comparative effectiveness of dried plasma HIV-1 viral load testing in Brazil using ViveST for sample collection

Comparative effectiveness of dried plasma HIV-1 viral load testing in Brazil using ViveST for sample collection

Author Zanoni, Michelle Autor UNIFESP Google Scholar
Cortes, Rodrigo Autor UNIFESP Google Scholar
Diaz, Ricardo S. Autor UNIFESP Google Scholar
Sucupira, M. Cecilia Autor UNIFESP Google Scholar
Ferreira, Denise Google Scholar
Inocencio, Lilian A. Google Scholar
Vilhena, Cintia Autor UNIFESP Google Scholar
Loveday, Clive Google Scholar
Lloyd, R. M. Google Scholar
Holodniy, M. Google Scholar
Institution VA Palo Alto Healthcare Syst
Universidade Federal de São Paulo (UNIFESP)
Lab Ctr Genomas
ICVC Charitable Trust HQ
Res Think Tank Inc
Stanford Univ
Abstract Background: Utilization of dried plasma for HIV-1 viral load testing would significantly decrease sample shipping costs.Objectives: To describe the precision and reproducibility of ViveST (R) (ST) as a transportation method for shipping specimens for HIV-1 viral load (VL) testing.Study design: Thirty clinical plasma samples were used to generate replicate samples with HIV VL values of 4 log(10), 3 log(10) and 2 log(10) copies/mL for reproducibility testing and an additional 299 samples with HIV VL <50 copies/mL (99); 1.7 log(10) to 3.99 log(10) (100); and 4 log(10) to 5.99 log(10)/mL (100) were used to compare ViveST to frozen plasma samples using the VERSANT (R) HIV-1 RNA 3.0 Assay. Results were compared using Student t-test, Pearson correlation and Bland-Altman analyses.Results: Meanintra-assay variance among frozen and dried plasma triplicates was 0.15 log(10) and 0.09 log(10) copies/mL respectively (n = 10, P = NS). Compared to frozen plasma, there was a mean reduction of 0.3 log(10), 0.27 log(10), and 0.35 log(10) copies/mL at the 4 log(10), 3 log(10), and 2 log(10) copy/mL samples respectively (n = 30, all comparisons, P<0.01). Overall correlation between 299 frozen and ViveST samples was r = 0.97, where 12 of 99 undetectable frozen VL were positive with ST, and 12 of 200 frozen detectable VL were undetectable with ViveST (mean VL 2.1, 1.9 log(10) copies/mL respectively).Conclusions: HIV-1 viral load results using ViveST were reproducible, correlated well with frozen plasma, though yielding minimally lower values. Our data suggest that dried plasma for HIV-1 VL testing using ViveST has promise for use in HIV clinical practice. Published by Elsevier B.V.
Keywords HIV
Viral load
Sample collection
Dried blood
Language English
Date 2010-12-01
Published in Journal of Clinical Virology. Amsterdam: Elsevier B.V., v. 49, n. 4, p. 245-248, 2010.
ISSN 1386-6532 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 245-248
Origin http://dx.doi.org/10.1016/j.jcv.2010.08.017
Access rights Closed access
Type Article
Web of Science ID WOS:000284105300004
URI http://repositorio.unifesp.br/handle/11600/33128

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