Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis

Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis

Author Castro, Leandro Mantovani de Autor UNIFESP Google Scholar
Berti, Denise A. Google Scholar
Russo, Lilian C. Google Scholar
Coelho, Veronica Google Scholar
Gozzo, Fabio C. Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Ferro, Emer Suavinho Autor UNIFESP Google Scholar
Institution Inst Biomed Sci
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Natl Inst Sci & Technol
Universidade Estadual de Campinas (UNICAMP)
Abstract Cells produce and use peptides in distinctive ways. in the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role.
Keywords intracellular peptide
mass spectrometry
MHC-I antigen presentation
peptidome
proteasome
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Financiadora de Estudos e Projetos (FINEP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Instituto de Investigacao em Imunologia iii-Instituto Nacional de Ciencia e Tecnologia
Grant number FAPESP: 04/04933-2
FAPESP: 04/14846
FAPESP: 2010/00828-0
Financiadora de Estudos e Projetos (FINEP): A-03/134
CNPq: 559698/2009-7
Instituto de Investigacao em Imunologia iii-Instituto Nacional de Ciencia e Tecnologia: 573879/2008-7
Date 2010-12-01
Published in Aaps Journal. New York: Springer, v. 12, n. 4, p. 608-616, 2010.
ISSN 1550-7416 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 608-616
Origin http://dx.doi.org/10.1208/s12248-010-9224-y
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288426100014
URI http://repositorio.unifesp.br/handle/11600/33096

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account