Bone marrow cell therapy prevents infarct expansion and improves border zone remodeling after coronary occlusion in rats

Bone marrow cell therapy prevents infarct expansion and improves border zone remodeling after coronary occlusion in rats

Author Santos, Leonardo dos Autor UNIFESP Google Scholar
Santos, Alexandra A. Autor UNIFESP Google Scholar
Goncalves, Giovana A. Google Scholar
Krieger, Jose Eduardo Google Scholar
Ferreira Tucci, Paulo Jose Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background: Since the cell therapy benefits for myocardial infarction are mainly related to infarct reduction by regenerating lost myocardium or increasing survival of tissues at risk, we evaluated the effects of bone marrow-derived mononuclear cells (MNC), implanted after the completion of necrosis, on infarct progression and cardiac remodeling.Methods: After 48 h of induction of myocardial infarction (MI), Lewis-inbred rats were injected with 6 x 10(6) cells (MI + MNC) or saline (MI). After six weeks, scar dimension, ventricular morphology and function were analyzed by echocardiography followed by histomorphology of the infarcted and border zones.Results: After therapy, the relative size of the infarct was smaller in MI + MNC (37 +/- 1% of the left ventricle) than in MI (43 +/- 1%). While the MI group exhibited parallel elongation of the infarcted (31.6 +/- 3.8% increase) and reminiscent ventricular portions (33.5 +/- 3.7%), MNC therapy preserved the initial infarct length. Infarcted walls were thicker (979 +/- 31 mm) in the MNC group than in the untreated group (709 +/- 41 mm), also demonstrating an absence of infarct expansion. in the border zones, MNC led to increased capillary densities and capillary/myocyte ratios. the cardiac systolic function remained depressed in MI, but improved by 19 +/- 5% in MI + MNC which reduced the incidence of pulmonary arterial hypertension (37.5% in MI and 6.25% in MI + MNC).Conclusion: MNC therapy prevented the infarct expansion and thinning related to cardiac remodeling and was associated with an improvement of border zone microcirculation: as a result, MNC therapy reduced typical MI dysfunctional repercussions. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
Keywords Stem cell
Capillary density
Myocardial infarction
Bone marrow
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2010-11-05
Published in International Journal of Cardiology. Clare: Elsevier B.V., v. 145, n. 1, p. 34-39, 2010.
ISSN 0167-5273 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 34-39
Access rights Closed access
Type Article
Web of Science ID WOS:000283727000019

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