Claudin-7 down-regulation is an important feature in oral squamous cell carcinoma

Claudin-7 down-regulation is an important feature in oral squamous cell carcinoma

Author Lourenco, Silvia Vanessa Google Scholar
Coutinho-Camillo, Claudia Malheiros Google Scholar
Cavicchioli Buim, Marcilei Elisa Google Scholar
Carvalho, Ana Carolina de Autor UNIFESP Google Scholar
Lessa, Roberta Cardim Google Scholar
Pereira, Claudia Maria Google Scholar
Vettore, Andre Luiz Autor UNIFESP Google Scholar
Carvalho, Andre Lopes Google Scholar
Fregnani, Jose Humberto Google Scholar
Kowalski, Luiz Paulo Google Scholar
Soares, Fernando Augusto Google Scholar
Institution Universidade de São Paulo (USP)
Hosp AC Camargo Fund Antonio Prudente
Universidade Federal de São Paulo (UNIFESP)
Ludwig Inst Canc Res
Hosp Canc Barretos
Abstract Aims:Claudins, a large family of essential tight junction (TJ) proteins, are abnormally regulated in human carcinomas, especially claudin-7. the aim of this study was to investigate claudin-7 expression and alterations in oral squamous cell carcinoma (OSCC).Methods and results:Expression of claudin-7 was analysed in 132 cases of OSCC organized in a tissue microarray. Claudin-7 mRNA transcript was evaluated using real-time polymerase chain reaction and the methylation status of the promoter was also assessed. Claudin-7 was negative in 58.3% of the cases. Loss of claudin-7 protein expression was associated with recurrence (P = 0.019), tumour size (P = 0.014), clinical stage of OSCC (P = 0.055) and disease-free survival (P = 0.015). Down-regulation of the claudin-7 mRNA transcripts was observed in 78% of the cases, in accordance with immunoexpression. Analysis of the methylation status of the promoter region of claudin-7 revealed that treatment of O28 cells (that did not express claudin-7 mRNA transcripts) with 5-Aza-2'-Deoxycytidine (5-Aza-dC) led to the re-expression of claudin-7 mRNA transcript.Conclusion:Loss of claudin-7 expression is associated with important subcellular processes in OSCC with impact on clinical parameters.
Keywords claudin-7
oral cancer
tight junction
tissue microarray
Language English
Date 2010-11-01
Published in Histopathology. Malden: Wiley-Blackwell Publishing, Inc, v. 57, n. 5, p. 689-698, 2010.
ISSN 0309-0167 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 689-698
Origin http://dx.doi.org/10.1111/j.1365-2559.2010.03685.x
Access rights Closed access
Type Article
Web of Science ID WOS:000284320300004
URI http://repositorio.unifesp.br/handle/11600/33065

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