Immunochemical and biological characterization of monoclonal antibodies against BaP1, a metalloproteinase from Bothrops asper snake venom

Immunochemical and biological characterization of monoclonal antibodies against BaP1, a metalloproteinase from Bothrops asper snake venom

Author Fernandes, I. Google Scholar
Assumpcao, G. G. Google Scholar
Silveira, C. R. F. Google Scholar
Faquim-Mauro, E. L. Google Scholar
Tanjoni, I. Google Scholar
Carmona, A. K. Autor UNIFESP Google Scholar
Alves, M. F. M. Autor UNIFESP Google Scholar
Takehara, H. A. Google Scholar
Rucavado, A. Google Scholar
Ramos, O. H. P. Google Scholar
Moura-da-Silva, A. M. Google Scholar
Gutierrez, J. M. Google Scholar
Institution Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Univ Costa Rica
Abstract BaP1 is a P-I class of Snake Venom Metalloproteinase (SVMP) relevant in the local tissue damage associated with envenomations by Bothrops asper, a medically-important species in Central America and parts of South America. Six monoclonal antibodies (MoAb) against BaP1 (MABaP1) were produced and characterized regarding their isotype, dissociation constant (K(d)), specificity and ability to neutralize BaP1-induced hemorrhagic and proteolytic activity. Two MABaP1 are IgM, three are IgG1 and one is IgG2b. the K(d)s of IgG MoAbs were in the nM range. All IgG MoAbs recognized conformational epitopes of BaP1 and B. asper venom components but failed to recognize venoms from 27 species of Viperidae, Colubridae and Elapidae families. Clone 7 cross-reacted with three P-I SVMPs tested (moojeni protease, insularinase and neuwiedase). BaP1-induced hemorrhage was totally neutralized by clones 3, 6 and 8 but not by clone 7. Inhibition of BaP1 enzymatic activity on a synthetic substrate by MABaP1 was totally achieved by clones 3 and 6, and partially by clone 8, but not by clone 7. in conclusion, these neutralizing MoAbs against BaP1 may become important tools to understand structure-function relationships of BaP1 and the role of P-I class SVMP in snakebite envenomation.(C) 2010 Elsevier B.V. All rights reserved.
Keywords Monoclonal antibodies
Metalloproteinase
BaP1
Hemorrhage
Snake venom
Neutralizing antibody
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
PAP (FUNDAP)
Vicerrectoria de Investigacion (Universidad de Costa Rica), NeTropica
Date 2010-11-01
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 56, n. 6, p. 1059-1065, 2010.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1059-1065
Origin http://dx.doi.org/10.1016/j.toxicon.2010.07.014
Access rights Closed access
Type Article
Web of Science ID WOS:000282253900023
URI http://repositorio.unifesp.br/handle/11600/33049

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