Glial cells modulate heparan sulfate proteoglycan (HSPG) expression by neuronal precursors during early postnatal cerebellar development

Glial cells modulate heparan sulfate proteoglycan (HSPG) expression by neuronal precursors during early postnatal cerebellar development

Author Araujo, Ana Paula B. Autor UNIFESP Google Scholar
Ribeiro, Maria Emilia de Oliveira Brenha Autor UNIFESP Google Scholar
Ricci, Ritchelli Autor UNIFESP Google Scholar
Torquato, Ricardo José Soares Autor UNIFESP Google Scholar
Toma, Leny Autor UNIFESP Google Scholar
Porcionatto, Marimélia Aparecida Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Investiga Inst Pesquisa
Abstract Cerebellum controls motor coordination, balance, eye movement, and has been implicated in memory and addiction. As in other parts of the CNS, correct embryonic and postnatal development of the cerebellum is crucial for adequate performance in the adult. Cellular and molecular defects during cerebellar development can lead to severe phenotypes, such as ataxias and tumors. Knowing how the correct development occurs can shed light into the mechanisms of disease. Heparan sulfate proteoglycans are complex molecules present in every higher eukaryotic cells and changes in their level of expression as well as in their structure lead to drastic functional alterations. This work aimed to investigate changes in heparan sulfate proteoglycans expression during cerebellar development that could unveil control mechanisms. Using real time RT-PCR we evaluated the expression of syndecans, glypicans and modifying enzymes by isolated cerebellar granule cell precursors, and studied the influence of soluble glial factors on the expression of those genes. We evaluated the possible involvement of Runx transcription factors in the response of granule cell precursors to glial factors. Our data show for the first time that cerebellar granule cell precursors express members of the Runx family and that the expression of those genes can also be controlled by glial factors. Our results also show that the expression of all genes studied vary during postnatal development and treatment of precursors with glial factors indicate that the expression of heparan sulfate proteoglycan genes as well as genes encoding heparan sulfate modifying enzymes can be modulated by the microenvironment, reflecting the intricate relations between neuron and glia. (C) 2010 ISDN. Published by Elsevier B.V. All rights reserved.
Keywords Cerebellum
Granule cell precursor
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Investiga Institutos de Pesquisa
Date 2010-11-01
Published in International Journal of Developmental Neuroscience. Oxford: Pergamon-Elsevier B.V., v. 28, n. 7, p. 611-620, 2010.
ISSN 0736-5748 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 611-620
Access rights Closed access
Type Article
Web of Science ID WOS:000282905900008

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