The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

Alternative title O papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratos
Author Bussuan, Luiz Antonio Maksoud Autor UNIFESP Google Scholar
Fagundes, Djalma Jose Google Scholar
Marks, Guido Google Scholar
Bussuan, Priscila Maksoud Google Scholar
Teruya, Roberto Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Mato Grosso do Sul (UFMS)
Moreira Salles Inst IMS
Abstract Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
Keywords Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
Language English
Date 2010-11-01
Published in Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 501-506
Origin http://dx.doi.org/10.1590/S0102-86502010000600008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000284779000008
SciELO ID S0102-86502010000600008 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/33018

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