Effects of magnesium ions on recombinant human furin: selective activation of hydrolytic activity upon substrates derived from virus envelope glycoprotein

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dc.contributor.author Izidoro, Mario A. [UNIFESP]
dc.contributor.author Assis, Diego M. [UNIFESP]
dc.contributor.author Oliveira, Vitor [UNIFESP]
dc.contributor.author Santos, Jorge A. N. [UNIFESP]
dc.contributor.author Juliano, Maria A. [UNIFESP]
dc.contributor.author Lindberg, Iris
dc.contributor.author Juliano, Luiz [UNIFESP]
dc.date.accessioned 2016-01-24T14:05:27Z
dc.date.available 2016-01-24T14:05:27Z
dc.date.issued 2010-09-01
dc.identifier http://dx.doi.org/10.1515/BC.2010.114
dc.identifier.citation Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 391, n. 9, p. 1105-1112, 2010.
dc.identifier.issn 1431-6730
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32901
dc.description.abstract Here we report a detailed analysis of magnesium (Mg(2+)) ion effects on furin hydrolysis of fluorescent resonance energy transfer decapeptide substrates derived from canonical R-X-K/R-R furin cleavage motifs within certain viral envelope glycoproteins and eukaryotic proproteins. Using virus-derived sequences a selective activation of furin by Mg(2+) ions was observed as a result of cooperativity between furin subsites. Furin hydrolysis of the peptides Abz-SRRHKR down arrow FAGV-Q-EDDnp (from measles virus fusion protein F(o)) and Abz-RERRRKKR down arrow GLFG-Q-EDDnp (from Asian avian influenza A, H5N1) was activated between 60- and 80-fold by MgCl(2). It appears that virus envelope glycoprotein mutations have been selected to increase their susceptibility to furin within cells, a location where Mg(2+) is present in adequate concentrations for activation. Both the pH profile of furin and its intrinsic fluorescence were modified by Mg(2+) ions, which bind to furin with a K(d) value of 1.1 mM. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 1105-1112
dc.language.iso eng
dc.publisher Walter de Gruyter & Co
dc.relation.ispartof Biological Chemistry
dc.rights Acesso restrito
dc.subject fluorescence en
dc.subject magnesium en
dc.subject peptidase en
dc.subject protease en
dc.title Effects of magnesium ions on recombinant human furin: selective activation of hydrolytic activity upon substrates derived from virus envelope glycoprotein en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Maryland
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ Maryland, Dept Anat & Neurobiol, Baltimore, MD 21201 USA
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1515/BC.2010.114
dc.description.source Web of Science
dc.identifier.wos WOS:000283654900010



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