Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases

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dc.contributor.author Niwa, Kazuki
dc.contributor.author Ichino, Yoshiro
dc.contributor.author Kumata, Shiho
dc.contributor.author Nakajima, Yoshihiro
dc.contributor.author Hiraishi, Yoshihiro
dc.contributor.author Kato, Dai-ichiro
dc.contributor.author Viviani, Vadim R. [UNIFESP]
dc.contributor.author Ohmiya, Yoshihiro
dc.date.accessioned 2016-01-24T14:05:27Z
dc.date.available 2016-01-24T14:05:27Z
dc.date.issued 2010-09-01
dc.identifier http://dx.doi.org/10.1111/j.1751-1097.2010.00777.x
dc.identifier.citation Photochemistry and Photobiology. Malden: Wiley-Blackwell, v. 86, n. 5, p. 1046-1049, 2010.
dc.identifier.issn 0031-8655
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/32895
dc.description.abstract Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. the green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). Mutant analyses of active site-substituted Pyrocoelia miyako luciferases showed that, although k(cat) was decreased by the mutations, the QY was not significantly affected. en
dc.description.sponsorship Japanese Society for the Promotion of Science
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 1046-1049
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Photochemistry and Photobiology
dc.rights Acesso restrito
dc.title Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Natl Inst Adv Ind Sci & Technol
dc.contributor.institution Univ Hyogo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan
dc.description.affiliation Natl Inst Adv Ind Sci & Technol, Natl Metrol Inst Japan, Tsukuba, Ibaraki, Japan
dc.description.affiliation Natl Inst Adv Ind Sci & Technol, Hlth Res Inst, Tsukuba, Ibaraki, Japan
dc.description.affiliation Univ Hyogo, Grad Sch Engn, Himeji, Hyogo, Japan
dc.description.affiliation Universidade Federal de São Paulo, Lab Bioquim & Biotecnol Sistemas Biol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Lab Bioquim & Biotecnol Sistemas Biol, São Paulo, Brazil
dc.description.sponsorshipID Japanese Society for the Promotion of Science: 21510238
dc.identifier.doi 10.1111/j.1751-1097.2010.00777.x
dc.description.source Web of Science
dc.identifier.wos WOS:000281638700009



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