Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases

Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases

Author Niwa, Kazuki Google Scholar
Ichino, Yoshiro Google Scholar
Kumata, Shiho Google Scholar
Nakajima, Yoshihiro Google Scholar
Hiraishi, Yoshihiro Google Scholar
Kato, Dai-ichiro Google Scholar
Viviani, Vadim R. Autor UNIFESP Google Scholar
Ohmiya, Yoshihiro Google Scholar
Institution Natl Inst Adv Ind Sci & Technol
Univ Hyogo
Universidade Federal de São Paulo (UNIFESP)
Abstract Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. the green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). Mutant analyses of active site-substituted Pyrocoelia miyako luciferases showed that, although k(cat) was decreased by the mutations, the QY was not significantly affected.
Language English
Sponsor Japanese Society for the Promotion of Science
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number Japanese Society for the Promotion of Science: 21510238
Date 2010-09-01
Published in Photochemistry and Photobiology. Malden: Wiley-Blackwell, v. 86, n. 5, p. 1046-1049, 2010.
ISSN 0031-8655 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1046-1049
Origin http://dx.doi.org/10.1111/j.1751-1097.2010.00777.x
Access rights Closed access
Type Article
Web of Science ID WOS:000281638700009
URI http://repositorio.unifesp.br/handle/11600/32895

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